FastQCFastQC Report
Wed 23 Nov 2016
G141-M10-Index-23_GAGTGG_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M10-Index-23_GAGTGG_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24555883
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG906180.3690276582601407No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA793620.32318935547949956No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA414900.16896154782949568No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC387760.15790920652293383No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT358390.14594873252979745No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA290950.11848484536271817No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA289420.11786177674816255No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC270730.11025056602525757No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG254960.10382847971706005No Hit
GGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG252690.10290405765494158No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG246120.10022852772184979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT56300.024.9668791
CTCGCTA105300.020.2180941
TCGCTAT114850.018.7623672
CCTCCCG314450.018.40821644
CGCTATG128050.016.676193
TGATCAA89250.016.2804554
CGCTGGT367300.015.66395336
TCCTCCC378500.015.50413943
ATATATC87700.015.45221812
GGTTCCT384700.015.05569440
TGGTTCC386850.014.9942439
GTTCCTC391300.014.87315241
GCGGGTA36450.014.8685241
GCTGGTT392850.014.76018337
CCCGAGT353600.014.7406511
GTTGATC102850.014.6534272
GATCAAA108750.014.6320555
GCCCGCT393850.014.62185533
CCGCTGG398000.014.45726335
CCCGCTG401200.014.34317834
Produced by FastQC (version 0.11.2)