FastQCFastQC Report
Wed 23 Nov 2016
G141-M11-Index-25_ACTGAT_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M11-Index-25_ACTGAT_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9993865
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG340020.34022873032605505No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA270600.27076611501155956No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG168330.16843333385031717No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA129790.12986967504564048No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC123910.12398606545115429No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA117920.1179923883302406No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT117710.11778225941615182No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC112130.11219883398464958No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA108210.10827642758832544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT19250.020.9638921
TAGATCG7000.018.11977822
CCTCCCG122100.017.77675444
CGCGATA3250.016.6003671
CTCGCTA44600.015.9676171
GTACGTA8650.014.7196311
TCGCTAT48600.014.6972982
TACGTTC10850.014.66247927
GGTTCCT146700.014.66247440
CGCTGGT146850.014.61845136
TCCTCCC151250.014.59318443
GTTCCTC149950.014.516644541
TGGTTCC149100.014.45528239
TACGTAT9250.014.2309492
CTGATCA126600.014.10633144
GCCCGGG55150.013.8913321
CTGGTTC154950.013.88200938
GCTGGTT155650.013.8059137
TAACGTA2653.45608E-1113.8050198
CCCGGGT14100.013.6217191
Produced by FastQC (version 0.11.2)