FastQCFastQC Report
Wed 23 Nov 2016
G141-M12-Index-27_ATTCCT_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M12-Index-27_ATTCCT_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8514244
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCG275510.32358715582968967No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA235430.27651309969505217No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG141750.16648571499712717No Hit
CCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACA123600.14516849646310348No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC110920.13027580604925112No Hit
GCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCA104420.12264154045855394No Hit
CCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCAC102810.12075059159685816No Hit
CGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACAC95200.1118126283437496No Hit
GGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG87950.10329748595412581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT15550.021.7842161
CTCGCTA41150.017.3552471
CCTCCCG106350.017.15767144
TCGCTAT45900.015.9820042
TACGTAT7850.015.1167352
GTACGTA7600.015.046481
GGTTCCT127100.014.47288640
TGGTTCC127150.014.38268939
GCCCGGG44250.014.3352981
CGCTATG50500.014.3125953
GTTCCTC130500.014.09581541
CGGCGAT1851.6088052E-713.9952981
TCCTCCC132750.013.95698243
CGCTGGT128900.013.9542736
CCGTTGA6050.013.9085511
CTGATCA118000.013.82445144
CTGGTTC133550.013.58128338
ACTGATC120750.013.4393643
GCTGGTT135050.013.41452237
GGCGAAT3101.8189894E-1213.2240531
Produced by FastQC (version 0.11.2)