FastQCFastQC Report
Wed 23 Nov 2016
DW-S1-1_S32_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-1_S32_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82126787
Sequences flagged as poor quality0
Sequence length20-51
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT30684863.7362791265656115No Hit
AGAGCAGTGGCTGGTTGAGATTTAA17976272.188843696028191No Hit
AGCAGTGGCTGGTTGAGATTT16676982.0306383104942363No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC12610411.5354807439380285No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT7929020.9654608794083227No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC5181970.6309719628992669No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4579470.5576097844908994No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC4266400.5194894571974428No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGG3989760.4858049542349684No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA3848950.4686595130039606No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC3820310.4651722220668392No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3812630.464237082597667No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG3452480.42038415553746183No Hit
ATTGATCATCGACACTTCGAACGCACT3275270.3988065428640232No Hit
TCTGTGACGTATGATGGATTCAATACATTT3087670.37596381312226423No Hit
TATTCTGTGACGTATGATGGATTCAATAC2888910.35176220883936443No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG2883480.3511010360115513No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACA2644460.3219972552926002No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGC2237400.27243242816743823No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG2067430.2517363792644171No Hit
CATTGATCATCGACACTTCGAACGCA1947250.23710290772729242No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC1926690.2345994614400293No Hit
GATCACAAGAAAGACGTGGTCCTGACAGAC1845410.22470256872462332No Hit
ATTGATCATCGACACTTCGAACGCA1836980.2236761070416647No Hit
TCTGTGACGTATGATGGATTCAATACATT1792990.21831975479571605No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1698910.20686429629835634No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGG1584430.19292487358600793No Hit
CCGTGGCGCAATGAAGGTGAAGGGC1546130.18826135253531834No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1501600.18283924829544349No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1488100.1811954484472892No Hit
AGCAGTGGCTGGTTGAGATT1487640.18113943748950023No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1390280.1692845965105149No Hit
CATGTCTTGGAGAATTCCTTAGTTCTTAA1383150.16841642666478598No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1371630.16701371746102767No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT1351060.16450905354424739No Hit
CACAAGAAAGACATGGTCCTGACAGACAG1346440.16394650870732347No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCG1276740.1554596309727787No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1229600.14971972542892734No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGA1222740.14888443158016154No Hit
CACAAGAAAGACGTGGTCCTGACAGACAG1208750.14718096788566684No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT1206170.14686681947024177No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1123720.1368274641013291No Hit
TATTCTGTGACGTATGATGGATTCAATACA1123320.13677875892064303No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG1111650.13535778527412742No Hit
ACATTGATCATCGACACTTCGAACGCA1085290.13214811386691652No Hit
AGCAGTGGCTGGTTGAGATTC1038940.1265044010549201No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGC972990.11847413438930711No Hit
CGTGATCGTATAGTGGTTAGTACTCTGT951390.11584405463226023No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC946190.11521088728334156No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT942410.11475062332585834No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC913170.11119027461770786No Hit
CTCGCCCGTCACGTTGAACGCACGTT837320.10195455473011503No Hit
AAAGACGTGGTCCTGACAGACAGACAATC832710.10139322752270828No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATT832280.10134086945347076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG930200.0653.9438545
CATACGT83950.0644.8815343
ATACGTC125000.0625.870244
TGAATAG100350.0616.3180537
CGTAAGT52100.0600.962235
GTAAGTG50050.0588.5425436
TACGTCT147350.0585.5968645
CGTAATA88050.0553.3266635
ACTCGTC67000.0549.3191543
CACTCGT47150.0537.6266542
GCATACG69650.0521.40742
TACTCGT21500.0508.3233642
TAGAGTA92050.0504.6969338
CCCGGTT30750.0496.367834
GTAATAT22700.0490.0088236
GAATAGC132700.0483.6994638
CGGCCCC341350.0482.9914230
TATACGT49050.0482.533943
TAGCATA21400.0475.4879540
CGTAAGA101200.0475.470535
Produced by FastQC (version 0.11.2)