FastQCFastQC Report
Wed 23 Nov 2016
DW-S1-3_S34_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-3_S34_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62295098
Sequences flagged as poor quality0
Sequence length20-51
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT37850006.075919488881774No Hit
AGAGCAGTGGCTGGTTGAGATTTAA34795035.5855165361486385No Hit
AGCAGTGGCTGGTTGAGATTT18602632.986210889338355No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT5146910.8262142873585334No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG4174870.6701763275177767No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3818430.6129583422438794No Hit
TCTGTGACGTATGATGGATTCAATACATTT2898480.46528219604052956No Hit
TATTCTGTGACGTATGATGGATTCAATAC2030750.32598873189026845No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1970350.3162929449119737No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA1899630.304940526781096No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1853360.297512976061134No Hit
ATTGATCATCGACACTTCGAACGCACT1843760.29597192382617327No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC1667280.2676422469068112No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1406570.2257914418884131No Hit
TCTGTGACGTATGATGGATTCAATACATT1313210.21080470890341968No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC1307190.20983834073107968No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1241360.19927089608238516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT1199740.19258979253873235No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1185690.19033439838235747No Hit
CACAAGAAAGACATGGTCCTGACAGACAG1150010.18460682090908662No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACA1079090.17322229752331395No Hit
TATTCTGTGACGTATGATGGATTCAATACA1078870.17318698174292943No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1070890.17190598207261829No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTG1008810.16194051095320533No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG997380.16010569563595517No Hit
CATGTCTTGGAGAATTCCTTAGTTCTTAA993490.15948124842824712No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTT979580.15724832795029875No Hit
GAGCAGTGGCTGGTTGAGATTTAATT825760.13255617641054196No Hit
AGAGCAGTGGCTGGTTGAGATTT797200.12797154601153368No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCG792940.12728770408226986No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC781670.1254785729689357No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA744700.119543916601592No Hit
CACAAGAAAGACGTGGTCCTGACAGACAG738470.11854383791161224No Hit
TTTTATCCGGTAAAGCGAATGATTAGAGGT726480.11661912788065604No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGA719640.11552112816324649No Hit
ATGGGGCTCTATGGCCGAGAACCAGATTTG712110.11431236531644914No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATT700620.11246791842273046No Hit
AGAGCAGTGGCTGGTTGAGATTTAATT700210.11240210265019569No Hit
GATCACAAGAAAGACGTGGTCCTGACAGAC696930.11187557646991743No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG682710.10959289284688178No Hit
AGAGCAGTGGCTGGTTGAGATTTA679400.10906155087836927No Hit
AGCAGTGGCTGGTTGAGATT674240.10823323530207787No Hit
AGCAGTGGCTGGTTGAGATTC670100.10756865652575102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGT55300.0645.070943
ATACGTC82650.0636.341844
ACGTCTG554950.0635.127945
TGAATAG126150.0625.805237
CGTAAGT38800.0562.901535
TACGTCT101300.0558.789945
GTAAGTG36850.0550.5436
GAATAGC149100.0547.329138
CCCGGTT29950.0526.8157334
GCATACG43750.0504.6403542
TAGAGTA71150.0502.514438
CACTCGT29000.0499.5312542
ACTCGTC41000.0499.2744443
CCCCGGT36800.0488.5912533
GTATACG17450.0487.591542
TCATACG27650.0482.8028342
TATACGT35150.0471.033343
AATAGCA222850.0465.388139
TAGCATA14800.0459.882940
CGGCCCC283450.0454.6378230
Produced by FastQC (version 0.11.2)