FastQCFastQC Report
Wed 23 Nov 2016
DW-S1-4_S35_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-4_S35_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78938703
Sequences flagged as poor quality0
Sequence length20-51
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT30717163.891267379956825No Hit
AGAGCAGTGGCTGGTTGAGATTTAA10516651.3322552309986648No Hit
AGCAGTGGCTGGTTGAGATTT10403571.317930191987066No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC4860750.6157625873331108No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC3085850.390917241191561No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGT2120850.26867048981030256No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG1900410.24074502465539624No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1899590.24064114658686497No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC1841440.2332746713611446No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1794050.2272712790834681No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1664940.21091555051265537No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1454820.1842974288543859No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1453650.18414921258587186No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1441010.18254797016363444No Hit
TCTGTGACGTATGATGGATTCAATACATTT1219600.15449962485448993No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1148170.14545083164084924No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG1067450.13522517591909256No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA970050.12288648826672512No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG944810.11968907064510548No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT941190.11923048697671154No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC933150.11821197518282No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT892730.11309154648765891No Hit
ATTGATCATCGACACTTCGAACGCACT872680.11055160103149908No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC861740.1091657155806069No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT848030.10742892494699337No Hit
AGCAGTGGCTGGTTGAGATT833770.1056224599991211No Hit
TATTCTGTGACGTATGATGGATTCAATAC808110.10237183653752203No Hit
GTGTTGTACACTTCCTGAAGATCAGAGACTG794120.10059957534392222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC127800.0659.8601744
ACGTCTG716550.0648.239945
CATACGT84100.0648.0212443
TACGTCT141350.0633.582145
TGAATAG157100.0624.291737
GCATACG64500.0546.950442
GTAAGTG56750.0539.641236
TAGAGTA103600.0529.183538
GAATAGC194800.0521.44338
CGTAAGT65800.0515.476435
TATACGT59950.0490.8584343
CACTCGT37750.0486.0317742
CGTAATA110850.0485.2217735
GTATACG29500.0479.1082842
TCGTTAG69300.0477.738734
TAGCATA20900.0476.3556840
ATCGTAA306450.0468.3988633
ACTCGTC54650.0466.390143
TAGATCT179650.0465.4014631
AGATCGT567750.0464.262231
Produced by FastQC (version 0.11.2)