FastQCFastQC Report
Wed 23 Nov 2016
DW-S1-5_S36_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-5_S36_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84033901
Sequences flagged as poor quality0
Sequence length20-51
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT39930604.751725140071743No Hit
AGCAGTGGCTGGTTGAGATTT37839544.502889851561217No Hit
AGAGCAGTGGCTGGTTGAGATTTAA28054373.3384586061284955No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT4107710.4888158173211547No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2374250.2825347831942254No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA2196520.26138498556671785No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT2060920.24524864078367611No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC2008750.23904043202754563No Hit
AGCAGTGGCTGGTTGAGATT1757380.20912750438659275No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC1614560.19213198254356892No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTG1592430.1894985215550091No Hit
AGCAGTGGCTGGTTGAGATTC1587920.18896183339150233No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC1216550.1447689546151142No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1194990.14220332339444766No Hit
ATTGATCATCGACACTTCGAACGCACT1192760.14193795430251416No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGT1170360.1392723634238996No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG1137090.1353132469716002No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1067380.12701778535784028No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT1042310.12403446556646229No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1005860.11969693040907382No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG965860.11493694669726211No Hit
TTTGGTGACTCTAGATAACCTCGGG939660.1118191573660254No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC910020.10829200943557292No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA866740.1031417070593926No Hit
AGAGCAGTGGCTGGTTGAGATTT849750.10111990397780057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGT79050.0696.3915443
ATACGTC117750.0693.915944
ACGTCTG743750.0664.38845
TGAATAG136650.0647.57837
TACGTCT144300.0616.9545
GTAAGTG52050.0556.362936
GCATACG63050.0547.965942
GAATAGC167750.0544.9634438
TAGAGTA98400.0531.0433338
CGTAATA107350.0524.630335
CGTAAGT61750.0519.4845635
CACTCGT41950.0512.989742
ACTCGTC61000.0503.2544643
TAGCATA21350.0501.3134840
GTATACG25850.0491.9270642
ATCGTAA281500.0482.132133
GTAATAT25650.0477.4852636
AGATCGT528650.0473.365531
TAGATCT168000.0472.8228531
TCGTATG75250.0472.341234
Produced by FastQC (version 0.11.2)