FastQCFastQC Report
Wed 23 Nov 2016
DW-S2-1_S37_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-1_S37_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79156819
Sequences flagged as poor quality0
Sequence length20-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT55139046.965797855014866No Hit
AGAGCAGTGGCTGGTTGAGATTTAA44309085.597632719424968No Hit
AGCAGTGGCTGGTTGAGATTT29153963.683063615782741No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC7604030.960628546733289No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG6498330.820943802706372No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC4178740.5279065092294828No Hit
TCTGTGACGTATGATGGATTCAATACATTT3829280.48375870182453895No Hit
TATTCTGTGACGTATGATGGATTCAATAC3795900.4795417562193852No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA2903870.366850264662606No Hit
TCTGTGACGTATGATGGATTCAATACATT2879830.3638132553052694No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2533870.32010760816449685No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC2248270.2840273306081186No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2165030.2735114962110845No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2068370.2613002930297136No Hit
CACAAGAAAGACATGGTCCTGACAGACAG2044260.25825444046709356No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1841800.23267736415734444No Hit
CATGTCTTGGAGAATTCCTTAGTTCTTAA1714970.2166547394988169No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1664550.21028510506466916No Hit
AGCAGTGGCTGGTTGAGATT1663920.21020551621711833No Hit
CACAAGAAAGACGTGGTCCTGACAGACAG1510380.19080857708544352No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC1461860.1846789725089888No Hit
ACTATACCTAAGACAGACTATGATAACTT1220880.15423560666327432No Hit
AGAGCAGTGGCTGGTTGAGATTT1219680.1540840088584156No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC1204570.15217513983223604No Hit
AGCAGTGGCTGGTTGAGATTC1203790.1520766012590779No Hit
GAGCAGTGGCTGGTTGAGATTTAATT1197570.15129081930389346No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1190320.1503749158995386No Hit
ATGGGGCTCTATGGCCGAGAACCAGATTTG1176090.14857721859692213No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGA1146240.14480622320106115No Hit
AGAGCAGTGGCTGGTTGAGATTTAATT1098940.13883074305954615No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT1089990.1377000760983081No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1065600.13461885071455434No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACA1054600.13322920417001596No Hit
GATCACAAGAAAGACGTGGTCCTGACAGAC1047100.1322817178896489No Hit
ATTGATCATCGACACTTCGAACGCACT1043550.13183324105027516No Hit
ACTGTAAGAGATGAAGAGTGCTCGGAATT1008310.12738131884759035No Hit
AAAGACGTGGTCCTGACAGACAGACAATC998920.12619506602457078No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATT997520.12601820191890226No Hit
ACTGTAAGAGATGAAGAGTGCTCCGAATT992630.12540044086410293No Hit
AGAGCAGTGGCTGGTTGAGATTTA953820.12049751519196343No Hit
CTGCTGTGTTTGGGACTGACCCTAGTCTG950800.12011599404973562No Hit
ACTATACCTAAGACAGACTATGATAACTTT943580.11920388059050224No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG940950.11887162873485355No Hit
TATTCTGTGACGTATGATGGATTCAATACA936410.11829808370647132No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGAC935090.11813132612112673No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG934000.11799362478171337No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC919800.11619971742421838No Hit
AGCAGTGGCTGGTTGAGATTTAATT887160.11207625713206085No Hit
AAATTCCATACTGTAAGAGATGAAGAGTG878930.11103654885373805No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT869120.10979723679901791No Hit
ATAGAAGATAATGGCAACTTTAGACTTTT854980.10801090933176584No Hit
AGTGGCTGGTTGAGATTTAATT852150.10765339117530734No Hit
ATCACAAGAAAGACGTGGTCCTGACAGA846120.1068916122058922No Hit
GCAGTGGCTGGTTGAGATTTAATT845010.10675138423639786No Hit
AAGACAGACTATGATAACTTTCTTATGGC818440.10339475617381745No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA816160.10310672034458586No Hit
CGCTGCCTCCAGGCCCGAGAATGAAGAATG811130.10247127287921966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG419900.01753.21445
TCACACG261050.01703.149541
TATCACA222100.01572.764439
ATATCAC223200.01529.264238
TGAATAG68500.01492.35337
ATCACAC294100.01439.364440
GTAAGTG46650.01413.749836
GTGCACA158100.01399.781540
CGTAAGT45450.01355.312135
GGTATAT61900.01350.724735
GTATATC67250.01335.247736
TACACGT31050.01329.263741
AATAGCA83550.01321.799639
TAAGTGC53700.01271.269737
TATATCA73550.01268.820737
TGCACAC183550.01253.194541
GAATAGC87200.01236.330638
CATACGT6900.01214.496343
CACGTCT558650.01149.563844
TAGATCA37650.01122.239637
Produced by FastQC (version 0.11.2)