FastQCFastQC Report
Wed 23 Nov 2016
DW-S2-2_S38_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-2_S38_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69901600
Sequences flagged as poor quality0
Sequence length20-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT55940918.002808233287936No Hit
AGAGCAGTGGCTGGTTGAGATTTAA30100854.306174679835655No Hit
AGCAGTGGCTGGTTGAGATTT15997092.288515570459045No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5115080.7317543518317178No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT4358920.6235794316582167No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3650940.5222970575780812No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG3135090.44850046350870365No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC3074090.4397739107545464No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA2831260.40503507788090687No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC2471750.3536042093457088No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTG2272580.32511129931217597No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC1930690.27620111699875255No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT1924740.2753499204596175No Hit
TCTGTGACGTATGATGGATTCAATACATTT1860620.2661770259908214No Hit
TATTCTGTGACGTATGATGGATTCAATAC1669780.23887579111207757No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGT1554330.22235971708802088No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT1526090.2183197523375717No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1521330.21763879510626366No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1501520.21480481133479062No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1450570.2075159939114412No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA1290320.18459091065154445No Hit
AGCAGTGGCTGGTTGAGATT1245430.17816902617393593No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC1215740.17392162697277314No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTT1197570.17132225871796927No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1187930.16994317726632865No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1116550.15973167996154594No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1088140.15566739530997858No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG1029280.1472469871934262No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG959990.13733448161415474No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG941820.13473511335935084No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC935080.13377090080913742No Hit
TCTGTGACGTATGATGGATTCAATACATT926450.13253630818178697No Hit
AGAGCAGTGGCTGGTTGAGATTT894860.12801709832106847No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATT876620.12540771598933356No Hit
AGAGCAGTGGCTGGTTGAGATTTAATT875920.12530757522002356No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATC862030.1233204962404294No Hit
AGCAGTGGCTGGTTGAGATTC851130.12176116140403082No Hit
GAGCAGTGGCTGGTTGAGATTTAATT846490.12109737116174736No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC793520.11351957608981768No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA784340.11220630142943794No Hit
ATTGATCATCGACACTTCGAACGCACT780150.11160688739599667No Hit
TATTCTGTGACGTATGATGGATTCAATACA754850.10798751387664947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG189350.01348.426341
ACGTCTG344450.01275.012545
TGAATAG89400.01245.026137
AATAGCA103600.01231.326539
TATCACA167850.01211.601939
ATATCAC165300.01174.608338
GTAAGTG40150.01147.153936
ATAGCAC170450.01138.323540
GTGCACA149950.01124.171340
ATCACAC222100.01109.197340
CGTAAGT40000.01097.318735
GAATAGC111450.01091.948438
TAGCACA189700.01054.445241
GGTATAT47300.01036.585335
TACACGT24450.0993.1441
GTATATC53350.0965.7712436
TGTATAG32600.0956.080337
TATATCA56650.0931.830437
CATACGT6650.0930.908543
TGCACAC188050.0920.933341
Produced by FastQC (version 0.11.2)