FastQCFastQC Report
Wed 23 Nov 2016
DW-S2-3_S39_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-3_S39_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68018092
Sequences flagged as poor quality0
Sequence length20-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCAGTGGCTGGTTGAGATTTAA1674375424.616618178586954No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATT746770910.97900393912843No Hit
AGCAGTGGCTGGTTGAGATTT49003587.204492004862471No Hit
AGAGCAGTGGCTGGTTGAGATTT4829700.7100610819838933No Hit
AGCAGTGGCTGGTTGAGATT4448500.6540171694319211No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC3619370.5321187192372288No Hit
AGAGCAGTGGCTGGTTGAGATTTA3486650.5126062636393859No Hit
GAGCAGTGGCTGGTTGAGATTTAATT2705970.3978309182798012No Hit
GCAGTGGCTGGTTGAGATTTAATT2605000.38298633839949525No Hit
AGCAGTGGCTGGTTGAGATTC2310960.3397566635653349No Hit
AGCAGTGGCTGGTTGAGATTTAATT2295130.3374293415934102No Hit
GAGCAGTGGCTGGTTGAGATTTAA2259660.3322145525634562No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT2248840.33062379932680264No Hit
GTGGCTGGTTGAGATTTAATT2094100.30787396976674974No Hit
AGTGGCTGGTTGAGATTTAATT2028790.29827211266084913No Hit
AGAGCAGTGGCTGGTTGAGATTTAATT2026390.2979192653625156No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC1992060.2928720787992701No Hit
GCAGTGGCTGGTTGAGATTTAA1944960.28594745056947496No Hit
AGCAGTGGCTGGTTGAGATTTAA1836980.2700722625386199No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT1834570.26971794504320995No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATT1783460.26220376778578264No Hit
AGAGCAGTGGCTGGTTGAGATTAA1476750.2171113532558367No Hit
CAGTGGCTGGTTGAGATTTAATT1338080.19672413039754189No Hit
AGTGGCTGGTTGAGATTTAA1286210.18909821816230896No Hit
ATTGATCATCGACACTTCGAACGCA1093770.16080574562426714No Hit
NGAGCAGTGGCTGGTTGAGATTTAA1016150.14939407591733092No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC949970.13966431166578447No Hit
AGAGCAGTGGGCTGGTTGAGATTTAA899690.13227216076569748No Hit
TGGCTGGTTGAGATTTAATT892040.1311474600022594No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT876810.128908349854918No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC875130.12866135674608456No Hit
AGAGCAATGGCTGGTTGAGATTTAA845360.12428458004967266No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC807000.11864490406464209No Hit
CAGTGGCTGGTTGAGATTTAA782530.11504733181871671No Hit
AGAGCAGTGGCTGGTTGAGATT779190.11455628599520258No Hit
ATTGATCATCGACACTTCGAACGCACT736260.10824472994626194No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC698600.10270796775657864No Hit
GCAGTGGCTGGTTGAGATTT692380.1017935051750643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG157950.02091.430441
TGAATAG41000.02082.10337
TATCACA128300.02054.67339
ATATCAC128200.02030.478138
ACGTCTG264950.02014.032345
GTAAGTG25150.01982.307536
GGTATAT35600.01938.997335
CGTAAGT26200.01862.063635
AATAGCA48100.01860.509939
TCTCGTA14850.01818.262337
ATCACAC175400.01811.471640
TGCCGTC26500.01804.837544
GTATATC40050.01778.728836
GTGCACA87650.01756.259640
TATATCA41850.01738.3537
GAATAGC51100.01715.973338
GCCGTCT32050.01714.142745
CGGAATA96300.01704.436534
CTCGTAT16450.01665.317538
CGTATGC16950.01665.11440
Produced by FastQC (version 0.11.2)