FastQCFastQC Report
Wed 23 Nov 2016
DW-S2-4_S40_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-4_S40_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71277501
Sequences flagged as poor quality0
Sequence length20-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATT42839456.010234561955252No Hit
AGAGCAGTGGCTGGTTGAGATTTAA29543714.144885775386542No Hit
AGCAGTGGCTGGTTGAGATTT24376973.4200090713056843No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4357130.6112910720593304No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT3466890.4863933150518282No Hit
TTTGGTGACTCTAGATAACCTCGGG2913300.40872645072110486No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC2693770.3779271105478291No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC2594080.3639409299717172No Hit
CGCGGCGTGGGAAATGTGGCGTACG2071530.29062887600394405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT1965330.2757293637441077No Hit
AGCAGTGGCTGGTTGAGATT1955690.2743769033092224No Hit
CCGTGGCGCAATGAAGGTGAAGGGC1585440.22243204065193029No Hit
AGCAGTGGCTGGTTGAGATTC1382560.19396864096006958No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC1285060.18028971021304463No Hit
ATTGATCATCGACACTTCGAACGCACT1275610.1789639061560253No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1138970.15979376156860495No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT1008140.14143874095698164No Hit
CGCGGCGTGGGAAATGTGGCGTACGGAAG1007010.14128020565704177No Hit
TTTGGTGACTCTAGATAACCTCGGGC995990.13973413574081392No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC973130.13652695259335762No Hit
AGTGGCTGGTTGAGATTTAATT958630.1344926500720052No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG920650.12916418043331793No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC875950.12289291679852805No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTT847740.11893514616905551No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATT847590.11891410166021393No Hit
ATTGATCATCGACACTTCGAACGCA846880.11881449098503045No Hit
AGAGCAGTGGCTGGTTGAGATTT839230.11774122103411006No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG824840.11572235115257477No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGT814500.11427168300976208No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC805890.11306372820225558No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCG766710.10756690249283571No Hit
AGAGCAGTGGCTGGTTGAGATTTAATT766670.10756129062381128No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA765370.10737890488051761No Hit
AGTGGCTGGTTGAGATTTAA752220.1055340029387394No Hit
GTCGCGGCGTGGGAAATGTGGCGTACG740560.10389814311812082No Hit
GAGCAGTGGCTGGTTGAGATTTAATT728800.1022482536249412No Hit
CGCGGCGTGGGAAATGTGGCGTACGG719840.10099119496347102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG261800.01743.776441
ATATCAC221250.01685.652538
TATCACA223100.01682.993839
ACGTCTG433150.01667.717545
TGAATAG53550.01665.239437
GTAAGTG45500.01636.862236
GGTATAT60850.01633.805535
CGTAAGT47550.01543.49535
CGGAATA171900.01525.981134
ATCACAC292950.01505.205240
GATCGGT139650.01497.217431
TATATCA71500.01442.272737
TCGTATG82900.01441.820234
TACACGT31500.01437.954641
ATCGTAA65750.01392.72933
GTATATC72950.01391.446936
GTGCACA159800.01376.151140
AATAGCA68150.01349.399739
TAAGTGC56900.01330.80537
TCGTAAG72000.01329.127334
Produced by FastQC (version 0.11.2)