FastQCFastQC Report
Wed 23 Nov 2016
DW-S2-5_S41_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-5_S41_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94746821
Sequences flagged as poor quality0
Sequence length20-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCAGTGGCTGGTTGAGATTTAA85289149.001794371549416No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATT63381156.6895278734470685No Hit
AGCAGTGGCTGGTTGAGATTT24241192.5585227814661984No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT12538161.3233330540979311No Hit
ATTGATCATCGACACTTCGAACGCA6243280.6589434805416848No Hit
TTTGGTGACTCTAGATAACCTCGGG5984910.6316739640267192No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5094330.5376781981951669No Hit
TTTGGTGACTCTAGATAACCTCGG4963170.5238349896721073No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATC4847360.5116118882764414No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC4659260.49175897943847635No Hit
ATTGATCATCGACACTTCGAACGCACT3282890.34649078094134683No Hit
AGAGCAGTGGCTGGTTGAGATTT2587600.27310678845889724No Hit
CTTGGATAACTGTGGTAATTCTAGAG2206570.2328911911461388No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTT2190940.23124153157603042No Hit
TTGGTGACTCTAGATAACCTCGGG1944510.205232215653969No Hit
CGCGGCGTGGGAAATGTGGCGTACG1912840.20188962329406282No Hit
GAGCAGTGGCTGGTTGAGATTTAATT1862980.19662717760208548No Hit
CCGTGGCGCAATGAAGGTGAAGGGC1857040.19600024363878127No Hit
AGCAGTGGCTGGTTGAGATT1825940.19271781160868712No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCT1822730.19237901396185103No Hit
AGCAGTGGCTGGTTGAGATTC1755550.18528853859909453No Hit
AGAGCAGTGGCTGGTTGAGATTTA1705540.18001026124137717No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGA1689660.17833421556170206No Hit
AGAGCAGTGGCTGGTTGAGATTTAATT1619360.1709144415515535No Hit
TTTGGTGACTCTAGATAACCTCGGGC1594650.16830643848198348No Hit
GCAGTGGCTGGTTGAGATTTAATT1565020.16517915677614134No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG1469430.15509016392222805No Hit
AGCAGTGGCTGGTTGAGATTTAATT1435260.15148371046665512No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATT1405840.14837859309284898No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1359200.1434560004920904No Hit
TTTGGTGACTCTAGATAACCTCGGGCC1344390.14189288736136063No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAAT1285660.1356942625019577No Hit
CTTGGATAACTGTGGTAATTCTAGAGC1212230.12794413440003438No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT1197710.126411629156402No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCG1179810.12452238371142817No Hit
CTTGGATAACTGTGGTAATTCTAGAGCT1162110.12265424715410768No Hit
CGCGGCGTGGGAAATGTGGCGTACGG1141030.12042937039544578No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTC1114570.11763666455890905No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTT1108440.11698967715233421No Hit
AGTGGCTGGTTGAGATTTAATT1088700.11490622994094968No Hit
GAGCAGTGGCTGGTTGAGATTTAA1071580.11309930915782387No Hit
GTGGCTGGTTGAGATTTAATT1038990.10965961591471232No Hit
CATTGATCATCGACACTTCGAACGCA963090.10164879305027026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG341900.01817.079641
ACGTCTG566550.01815.565845
TATCACA283150.01780.714139
ATATCAC286000.01763.757138
GTAAGTG37600.01706.35836
GGTATAT81600.01645.369135
CGTAAGT39200.01626.770935
TGAATAG43650.01623.890137
CGGAATA226300.01596.336834
GATCGGT182650.01593.270131
GTATATC86200.01578.212636
ATCACAC390550.01544.217840
TATATCA90750.01523.216137
TACACGT49450.01479.726941
AATATCA248200.01450.549637
TCGTATG75900.01440.240634
GTACACG49750.01418.338640
GGAATAT258650.01365.855335
TCGTAAG63050.01329.32934
GAATATC269350.01325.582636
Produced by FastQC (version 0.11.2)