FastQCFastQC Report
Wed 12 Oct 2016
DW-S1-1_S32_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-1_S32_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88030048
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG25661992.915139839523886Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT12819161.4562254924591203Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA11114101.2625348108409529Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11049901.2552418465113184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG9414901.0695098110136212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC9009611.0234698497494854Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC6032860.6853182676896871No Hit
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG4521470.5136280284659165Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG4246660.4824102788175238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCTG3665960.41644416688265357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA3555960.4039484336075791Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3474410.3946845513477398No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTCT3385240.3845550555646636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT3223970.36623517460765215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTG2845170.32320441311130493Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTTTGAACT2746000.31193894157594915Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT2484850.28227293480517013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGAA2482220.28197417318232065Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2459660.2794114118851781No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTCT2351710.267148553639321Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC2169840.24648856263261382No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCTG2155890.2449038764581839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG1891270.21484368610136395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTCT1777380.20190605825865277Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1717450.19509815557524177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG1650670.1875121095015193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG1647900.1871974442181379Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCT1629390.18509475310066853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCTG1389500.15784383077923575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1377780.15651246719756418Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAA1311510.14898435588720796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1304020.1481335100487506No Hit
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1264270.14361800643343964Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC1204400.1368169196045423No Hit
CACAAGAAAGACATGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCTG1178280.13384975093958826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAACT1168920.13278647763545467Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT1132540.12865379784866185Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC1116830.12686917994183078No Hit
ACAAGAAAGACGTGGTCCTGACAGACAGAAGATCGGAAGAGCACACGTCTG1080880.12278534711238599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTG1078950.12256610379219605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1064860.12096551395723425No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCTG1062090.12065084867385281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGTCTTGGAGAATTCCTTAGTTCTTAAAGATCGGAAGAGCACACGTCTG1052030.11950805706705965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTCT1042350.1184084325388531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCTGAAGAGCACACGTC978620.11116885906957588No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT956850.10869583985686342No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG956180.10861972948146069No Hit
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG949180.10782454645486504Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATTGGAAGAGCAC929670.10560825776216776No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG920720.1045915594638776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC4331200.043.589391
GAGAGCA4455700.042.277272
CGCACTA379450.041.59684822
ACGCACT823000.041.5744221
CGCACTT442350.040.90509822
GACCGCC255650.040.9048541
CTTGCGG327450.040.60513326
TTGCGGC326700.040.5600727
ACTTGCG331800.040.43907525
GAGATAG2886600.040.1640313
TGAGATA2901650.039.99741412
GCCGTGA631800.039.8147541
CGCCCCG307700.039.5673036
CACTTGC385850.039.5309824
CTAAACC118000.039.400051
TCGTAAG2136600.039.34432634
GCGCCCC310550.039.333975
CGACGAA413350.039.087961
ATCGTAA2245800.039.0585933
GCTACGC840150.038.910859
Produced by FastQC (version 0.11.2)