FastQCFastQC Report
Wed 12 Oct 2016
DW-S1-5_S36_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-5_S36_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88891368
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG34056483.831247146517083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG19339092.175586947880024Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT19098682.1485415771754126Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG12863991.4471585137490517Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC7745700.8713669475758322Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7504400.8442214546636293Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTTTGAACT5764170.6484510397005028Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3176180.35731028461616204No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG2238460.251819726747821Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG2041650.22967921924657522Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT1887960.21238957645471268No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1818990.20463066785067363No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1753830.19730037229261677No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1728790.1944834508565556Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC1700540.19130541449198982No Hit
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1502140.16898603697942863Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTTTGAACTCCAG1448780.16298320439842934No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG1394580.1568858744529615Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT1319640.14845535958002132No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA1081140.12162485788271364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC1041440.11715873244295216No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTCT990340.1114101427711181Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGAA919330.10342174056765557Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTC906190.10194353179489825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC5404200.043.1003531
GAGAGCA5488150.042.3652232
CCCGCTA241300.042.1329826
GCGACCT617100.040.742182
CGACGAA551950.040.3855821
GATTTAG4405400.040.02796617
CCGCTAG256850.039.6342227
CGCACTA150400.039.36971722
ACCCGCT535250.039.3210425
ATTTAAA3384900.039.2402820
GCGACCC616800.039.2347622
CGACCTC644050.039.1734283
CGCCCCG414700.039.1335076
GCGCCCC418700.038.893515
GAGATAG2274600.038.87006813
CGCGACC656200.038.7962841
ACGCACT336750.038.6406921
TGAGATA2309850.038.3796512
TGCGCCC424900.038.3365754
ATTTAGA4607300.038.30034618
Produced by FastQC (version 0.11.2)