FastQCFastQC Report
Wed 12 Oct 2016
DW-S2-2_S38_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-2_S38_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73050900
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG50759956.948572844413964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT27590783.776925404067575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG9590291.3128229768558635Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG4315590.5907647955055996Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA4231600.5792673327775565No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3623440.4960157917287809No Hit
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3443050.4713220507892442Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC3386440.4635726596113121Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3008930.4118949937646217No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTCT2840130.38878781780922617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG2818760.3858624602845413Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2608790.3571194879186978No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA2346310.32118837687146906Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT2037210.2788754142659433No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG1782620.2440243720474354Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT1703910.2332496930222626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC1683560.23046396416745038No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTCT1567530.21458051851517232Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTGA1450410.1985478618333244Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1432300.19606876848882082Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCTG1427600.19542538148058408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGA1408840.19285730908174986No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1301530.17816755166603013No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC1217490.16666324439534627No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTCT1190050.16290695939406633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1186160.16237445397661082Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGATCACACGTCTGAACT1136750.15561067693895628Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCTG1121210.15348339308619058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTGAACTCCAGTCAC1075150.14717820040547072Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TTTGGTGACTCTAGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTG1069560.14641298053822746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTTAACTCCAGTCAC1065100.14580244733466666Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT999040.13675943759762027No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA975200.13349595966647912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT943490.1291551507236735No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC926070.1267705120676131No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGT921640.12616408558963682No Hit
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG889480.12176167576306382Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT870060.11910325540137083Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC854500.11697323373154882No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA837710.11467483631276275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG831600.113838433202055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCTG812120.111171799389193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAAC806370.11038467698549916Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCC780800.10688437787898576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA775210.1061191580117425Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCAGATCGGAAGA770780.1055127315337662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC7346800.044.267141
GAGAGCA7526000.043.1929662
CCCGCTA213950.042.690926
GCGACCT1003550.042.0757642
CGACCTC1018150.041.5502553
CGCGACC1025650.041.3794141
ACCCGCT887500.041.24209625
GCGACCC966100.041.10218422
ATCGTAA1574100.041.07451633
TCGTAAG1576350.041.0330234
GACCCGC961500.040.88237824
TCAGACG1021250.040.81521613
GGCGACC977300.040.69157421
TGGCGAC981850.040.53049520
GTGGCGA982600.040.49713519
AGATCGT1679900.040.41417731
ATTTAAA3584400.040.369120
TAAGAGC1563050.040.32599337
TAGATCT518200.040.32154531
ACGCACT502450.040.10674721
Produced by FastQC (version 0.11.2)