FastQCFastQC Report
Wed 12 Oct 2016
DW-S2-4_S40_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-4_S40_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80285373
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG38516184.797409361229473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT27049133.3691230406315733Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC14810651.8447507243940935Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG14136701.7608064173781695Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC13049191.6253508593651298Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG6904640.8600121967422384Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTTAACTCCAGTCAC4976900.6199012116441185Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTGAACTCCAGTCAC4206730.5239721561734539Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3502590.43626751288805743No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2816540.3508160820277935No Hit
TTTGGTGACTCTAGATAACCTCGGGAGATCGGAAGAGCACACGTCTGAACT2600200.3238697041365181Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA2560030.31886630208468986Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2524570.3144495573309474Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG2366930.2948145984200634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAACT1911490.23808695514187872Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1862600.23199742747660898Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1829810.2279132464141382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC1695360.2111667339454224No Hit
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG1688280.21028487966294934Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAACT1467250.1827543355873803Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG1420350.17691267374444408Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGATCACACGTCTGAACT1185850.1477043645297631Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1142860.14234971543322095Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1134590.14131963988010618No Hit
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC1104040.13751446356237268Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGAA1064110.13254095487605194Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTG1016270.12658221068487782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGCTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC1008840.12565676191103953TruSeq Adapter, Index 9 (100% over 37bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTGA958770.11942025853202425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC944320.11762042881708977Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGCGTGGGAAATGTGGCGTACGGAAGAGATCGGAAGAGCACACGTCTG930640.1159165069831587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA913020.11372183573214513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA911500.11353251108393056TruSeq Adapter, Index 7 (97% over 35bp)
TTTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA873520.1088018859923588Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT851720.10608657195875516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGGCTGGTTGAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC837950.10437144011275878Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTTTGAACTCCAG817250.10179313733773149No Hit
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA810440.10094491309145441Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC5665700.043.5333021
ATCGTAA1571250.043.10801733
TCGTAAG1583950.042.8078534
GAGAGCA5773500.042.7187962
TAAGAGC1551850.042.62133437
CGTTTTA683550.042.53800634
ACGTTTT684200.042.4534233
AGATCGT1666200.042.34374231
CCCGCTA223150.042.2211926
TTTTAAC675350.041.9026336
GTTTTAA676450.041.85075435
TTTAACT685750.041.25545537
CGCCCCG247450.041.047686
GCGACCT410400.040.8087922
GCGCCCC251200.040.542375
GTAAGAG1646250.040.1294536
CGTAAGA1647500.040.11094735
TGCGCCC255800.039.8082584
GGGCGTC258800.039.5762212
CCGCTAG239950.039.5091827
Produced by FastQC (version 0.11.2)