FastQCFastQC Report
Wed 12 Oct 2016
DW-S2-5_S41_L008_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S2-5_S41_L008_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100295421
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT77963917.773426665211367Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG57252155.708351331413226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG14808571.4764951233416728Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC10322421.0292015225700086No Hit
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8866030.8839915034605618Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC7771800.7748908098207196Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG6229880.6211529836441885Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAACT5454100.5438034902909475Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGAGATCGGAAGAGCACACGTCTGAACT5312500.5296851986891804Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGAGATCGGAAGAGCACACGTCTGAACTC4695240.46814101313757883Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG4448550.44354467588305946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA4411910.4398914682256531Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC4133970.4121793356847268No Hit
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGATCACACGTCTGAACT3270020.3260388128786059Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGAA2729980.2721938821115273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTGAACTCCAGTCAC2576590.25690006326410453Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTTAACTCCAGTCAC2282720.22759962291797947Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCC2260810.22541507652677384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAAC2090080.2083923651908296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG2086040.20798955517620293Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGGTGACTCTAGATAACCTCGGGAGATCGGAAGAGCACACGTCTGAACTC1839990.1834570294091492Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAAC1769550.17643377756996503Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCTGAACT1753920.17487538139951572Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1738090.1732970441392334No Hit
AGCAGTGGCTGGTTGAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1736780.17316643000082727Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGTGGCGCAATGAAGGTGAAGGGCAGATCGGAAGAGCACACGTCTGAACT1707730.17026998670258336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1664170.165926817336955Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTCT1654560.16496864797047914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCAGTGGCTGGTTGAGATTTAAGATCGGAAGAGCACACGTCTGAACTC1608680.1603941619627879Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCTGAAGAGCACACGTC1595090.15903916490863526No Hit
TTTGGTGACTCTAGATAACCTCGGGCAGATCGGAAGAGCACACGTCTGAAC1505610.15011752131734907Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGAAGATCGGAAGAGCACACGTCTG1504340.1499908953969095Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTC1486550.14821713545626375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAA1427440.14232354635611927Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGAGATCGGAAGAGCACACGTCTGA1388630.13845397787402478Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG1358310.1354309086553413Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGA1340110.1336162694805379Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACT1293350.12895404267758145Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTGA1219490.12158979820225291Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1199610.11960765387285228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCAGATCGGAAGAGCACACGTCTGAA1155930.1152525198533241Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACACGTCTGA1104890.11016355372794137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGGCGTGGGAAATGTGGCGTACGGAGATCGGAAGAGCACACGTCTGAAC1082610.10794211632054469Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1075660.10724916344884779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCAGATCGGAAGAGCACACGTCTGAA1067180.10640366123992838Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC1065820.10626806182906397No Hit
AGCAGTGGCTGGTTGAGATTCAGATCGGAAGAGCACACGTCTGAACTCCAG1055330.10522215166732288Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAACTCCA1033340.10302962884018403Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACTC1016970.10139745063735262Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC8356100.044.0930861
GAGAGCA8599150.042.826582
ATCGTAA1341350.042.81439633
TCGTAAG1350850.042.56308734
TAAGAGC1325550.042.2950937
GACCGCC327800.042.186391
CGCACTA377500.041.44118522
AGATCGT1453350.041.3889731
ATTTAAA9974450.040.80097220
CGTTTTA347500.040.6003134
ACGTTTT348200.040.4203633
ACGCACT509500.040.39046521
TTTAAAG10184150.039.9893421
CGCCCCG179550.039.981246
GTAAGAG1403550.039.9063336
TTAAAGA10228450.039.86680222
CTAAACC148550.039.8048321
TAGATCT416550.039.6566531
GAGATAG2669250.039.55375313
TTTGGTG2341050.039.3513371
Produced by FastQC (version 0.11.2)