FastQCFastQC Report
Thu 12 Feb 2015
G111_M9.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG111_M9.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22039079
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC2450.020.64573316
CACGACG3250.014.88709215
CGACGCT3250.014.88675417
GATCGGA7100.012.3906151
TCTCGTA7250.011.53094743
CTCGTAT7750.09.9353444
ATCGGAA10000.08.5770922
TATCTCG11650.07.55509941
ACACGAC8800.07.49737314
CGTCTGA13050.07.07794816
TACACGA9800.06.95673113
ATCTCGT12800.06.70392642
ACACGTC14550.06.34827713
TCGGAAG14300.05.9979673
GACGCTC9251.1908924E-85.943701718
CTACACG11807.8216544E-115.77742712
ACGTCTG15800.05.5676715
Produced by FastQC (version 0.11.2)