FastQCFastQC Report
Wed 12 Oct 2016
DW-S1-3_S34_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-3_S34_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67137800
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG32149854.788636207918639Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT24714563.6811691774231505Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG10525141.5676921197894478Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC10343241.540598589766123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC7043831.049160085674538Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTTTGAACT5975130.8899800112604226Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG5346200.7963025300203461Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3948570.5881291910071524No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTCT3606470.5371742892975343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2439810.363403328676245No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTCT2236990.3331938192791542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG2064570.3075123105016846Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1719970.2561850403200582Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTCT1693520.25224538188621015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1640130.24429308079800052No Hit
TATTCTGTGACGTATGATGGATTCAATACAGATCGGAAGAGCACACGTCTG1543230.22986007882295814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAGATCGGAAGAGCACACGTCTG1459180.21734105079403854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1417900.2111925025842372No Hit
ATTGATCATCGACACTTCGAACGCACTAGATCGGAAGAGCACACGTCTGAA1414050.21061905513734439Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG1124820.16753900187375814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGATCACACGTCTGAACT1077180.16044314827116765Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACTTCTGAACTCCAG1054730.157099279392533Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCTGTGACGTATGATGGATTCAATACATTAGATCGGAAGAGCACACGTCTG1035170.1541858684675399Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACAAGAAAGACATGGTCCTGACAGACAGAGATCGGAAGAGCACACGTCTG1024920.1526591577323058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC996530.14843054136417932No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACAC989160.14733279910869881No Hit
GATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTC901000.13420159731179754No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT868360.12933995454125694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTTTGAACTCCAGTCAC866840.1291135545102759Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT847970.1263029172835571No Hit
TATTCTGTGACGTATGATGGATTCAATACAAGATCGGAAGAGCACACGTCT841880.12539582768574484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT834680.12432340648636088No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATTGGAAGAGCAC798130.11887937942559929No Hit
GAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTGAAC754890.11243889433374342Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATGTCTTGGAGAATTCCTTAGTTCTTAAAGATCGGAAGAGCACACGTCTG754610.1123971890648785Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC680910.10141976651007331No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTG679700.10123954016962129Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC4838250.043.9132041
GAGAGCA4961500.042.7539332
CCCGCTA134550.042.6207626
CGCGACC614950.042.1013641
ACGCACT555200.040.98607321
CGCACTA213250.040.8792222
ATTTAAA4004400.040.80247520
CGCACTT343900.040.62368422
CCGCTAG143600.040.24741427
CTTGCGG267100.040.1687726
ACTTGCG268700.040.1473325
GAGATAG2450900.040.03951613
GCGACCT644450.039.9252432
TTTAAAG4096700.039.91338321
TGAGATA2463100.039.8866112
TTGCGGC268900.039.8239627
CGCCCCG154900.039.6329276
GCGCCCC156150.039.459085
TGCGCCC157850.039.0198674
CACTTGC312600.038.88360224
Produced by FastQC (version 0.11.2)