FastQCFastQC Report
Wed 12 Oct 2016
DW-S1-4_S35_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDW-S1-4_S35_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81454432
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGAGCACACGTCTG25924293.1826739642601645Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTCTGAACT7712550.9468545554402737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTCTGAACTCCAG6101040.7490126504104774Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4357490.5349604549449194Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGA4288410.5264796395609266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGGCTGGTTGAGATAGATCGGAAGAGCACACGTCTTAACTCCAGTCAC2984560.3664085460690463Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGCAGTGGCTGGTTGAGATTTAGATCGGAAGAGCACACGTTTGAACTCCAG2697870.3312121800812508Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT2585360.31739954923508645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAATAGCACACGTCTG1699290.20861848254002924Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC1662390.20408834230161962No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTAGATCGGAAGAGCACACGTC1649050.2024506168062163No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACGAGATCGGAAGAGCACACGTCT1640790.2014365528937701Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATCAGATCGGAAGAGCACACGTCTG1582020.19422147587991284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCAGTGGCTGGTTGAGATTTAAAGATCGGAAGAGCACACGTTTGAACT1517320.18627838445917835Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATTAGATCGGAAGATCACACGTCTG1397250.17153762732026662Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG1366060.16770849252254316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGT1256450.1542518889579882No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1167930.14338446310693076No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1125210.13813981294473948No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1105270.13569181846360429No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTC998810.12262193418769406No Hit
TCTGTGACGTATGATGGATTCAATACATTTAGATCGGAAGAGCACACGTCT952600.11694882360729002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTG886640.10885104447109765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCACAAGAAAGACGTGGTCCTGACAGACAAGATCGGAAGAGCACACGTCT863240.105978272612594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGAGCAGTGGCTGGTTGAGATTTAATAGATCGGAAGAGCACACGTCTGA843480.10355237637652424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT821480.10085147975741823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGC4373800.042.328831
GAGAGCA4378900.042.203182
CCCGCTA103900.041.1844326
ATTTAAT4171450.039.61413622
CGACGAA433100.039.5311281
TTTAATT3840900.039.5032123
TTAATTA3852350.039.3752824
TAATTAG3867100.039.2522425
GGTCGAC375250.038.99307621
CGCCCCG428450.038.8856966
ACGCACT251750.038.738921
GCGACCT297350.038.7237132
CGCACTA105500.038.575722
ATTTAAA1310950.038.3942120
GCGCCCC434900.038.3911825
GTCGACC377100.038.0142922
TACAAGC400600.037.927144
CGAGCGC432250.037.87956210
AGGTCGA388100.037.8701220
TGCGCCC442650.037.795254
Produced by FastQC (version 0.11.2)