FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP17_CGATGT_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP17_CGATGT_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4580.1832TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA250.00546286455.785964
CTCGTAT503.646892E-837.201842-43
CGATCAC651.504115E-435.760233
TATGCCG653.653131E-728.61390546-47
ATCTCGT902.3868233E-723.25345240-41
TGGGATT1754.8546262E-621.2517952
CACGATC1100.003245489421.1395021
TGCAACT1100.003253030621.1310462
CGTATGC906.205317E-620.665644-45
GAATTGG2351.4091893E-719.7822554
CGATGTA959.867908E-619.58577334-35
ACGATCA1200.00538340819.3701272
AGTCTAA1200.00538340819.3701276
TAATGTA1550.00110879417.995474
TATCTCG850.002055198216.41584438-39
GGAATTG2556.714705E-616.4076373
ACCGATG1155.0997973E-516.17955232-33
AATTGGA2053.910259E-415.8740545
TGCCGTC1207.356329E-515.50075148-49
ATTGGAC1800.003013928415.49616
Produced by FastQC (version 0.11.2)