FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP18_ACAGTG_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP18_ACAGTG_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2950.11800000000000001TruSeq Adapter, Index 5 (100% over 50bp)
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT2900.116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA906.708906E-736.1625442
CGATCAC951.0241529E-634.259253
CGGACAA700.0092642926.589671
ACACTAA700.00930052226.5684018
CACGATC1258.636805E-626.0578781
CCACGAT900.001006799825.8510681
CTCTCAT1151.4694915E-424.2775271
CGTATGC1254.984031E-1022.31745544-45
AACCGTG550.003251156821.13818226-27
ATCTCGT1251.20962795E-820.45766840-41
CTCGTAT1251.20962795E-820.45766842-43
TATGCCG1251.20962795E-820.45766846-47
GATCTCG1356.067821E-717.22370538-39
GCCGTCT1255.577336E-616.73809248-49
TCACAAG2254.2281063E-516.5314486
TAGTATG2003.2365628E-416.2731469
ATTGGAC2305.103602E-516.172076
CATTGAA1800.00301116515.4982345
GAGCACA3302.873221E-715.4982339
GGATAAT2155.61022E-415.137813
Produced by FastQC (version 0.11.2)