FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP20_CAGATC_L007_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP20_CAGATC_L007_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC3210.1284TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC300.004383451330.998230-31
CGTGACC650.00647016628.6194482
TAATGTA700.00929798626.5698874
CCCGCCT900.001005295825.857691
TATGCCG751.2742967E-624.7985646-47
AGGCTAA1151.476937E-424.259465
CTTGAGT1303.3646988E-421.4817751
CACGAAG1100.003230576221.1562941
GTTTAGG1150.00417959520.2364541
CTAAGGC1150.004203886320.2162178
CTCGTAT959.902635E-619.5778142-43
ATGTAGC1200.0053773919.3738756
CGTATGC1052.3453711E-517.71325744-45
CGGGAAG1902.1707836E-417.147731
ATCTCGT1103.496868E-516.9081140-41
AATGTAG1650.001683568616.908115
GGAATTG1700.00205553816.4108123
ATGCCGT1003.2432986E-416.27405546-47
GCCGTCT1054.701617E-415.499148-49
TGCCGTC1251.0429367E-414.87913748-49
Produced by FastQC (version 0.11.2)