FastQCFastQC Report
Fri 5 Jun 2015
Waxman-TP20_CAGATC_L007_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameWaxman-TP20_CAGATC_L007_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT405.9782626E-446.49481
ATGTAAG600.00438045330.9965326
ATTGGAC600.00438045330.9965326
CGGGCTG1000.001868720223.24742
ATTGTGA1405.587349E-419.9263448
CAGATTC1405.587349E-419.9263443
ATCTACA1200.005379061719.3728336
GGATTGA1200.005379061719.3728334
CTCACAT1250.006809256518.5979211
CCGGGCT1300.00853731117.8826161
GATTGTG1300.00853731117.8826167
GGAATTG2102.3388478E-517.7123053
AAATTAG1600.001370565817.435554
ATTCAGC1900.00431499514.6825686
ACAGCCG1200.001293031513.56098336-37
CTAGAAT2150.00978353512.9752931
AATATTC2700.003126456612.0542073
ATATTCA2750.0035866311.835044
TGGAATT3300.001085169111.2714662
TTGCAAA2900.0053316411.2228839
Produced by FastQC (version 0.11.2)