----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: KI4 Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo Sample_Labels_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Apr 30 05:12:25 EDT 2019 Running on node : scc-ka1 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq Current job ID : 5782401 Current job name : Step_08a_KI4 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/5782401.1.linga Loading required modules... List files in scratch directory: total 372M drwx------ 2 kkarri waxmanlab 4.0K Apr 30 05:23 . drwxrwxrwt. 67 root root 124K Apr 30 05:20 .. -rw-r--r-- 1 kkarri waxmanlab 0 Apr 30 05:23 KI4_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 291M Apr 30 05:16 KI4_primary_unique.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Apr 30 05:16 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view KI4'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > KI4_HTSeq.out Ending samtools and htseq-count List files in scratch total 827M drwx------ 2 kkarri waxmanlab 4.0K Apr 30 05:29 . drwxrwxrwt. 59 root root 124K Apr 30 05:45 .. -rw-r--r-- 1 kkarri waxmanlab 230K Apr 30 05:47 KI4_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 291M Apr 30 05:16 KI4_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 456M Apr 30 05:29 KI4_sorted.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Apr 30 05:16 genes.gtf ========================================================== Finished on : Tue Apr 30 05:47:29 EDT 2019 0 hours, 35 minutes and 5 seconds elapsed. ==========================================================