----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: KI5 Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo Sample_Labels_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Apr 30 05:08:42 EDT 2019 Running on node : scc-kb4 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq Current job ID : 5782409 Current job name : Step_08a_KI5 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/5782409.1.linga Loading required modules... List files in scratch directory: total 386M drwx------ 2 kkarri waxmanlab 4.0K Apr 30 05:19 . drwxrwxrwt. 63 root root 164K Apr 30 05:15 .. -rw-r--r-- 1 kkarri waxmanlab 0 Apr 30 05:19 KI5_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 306M Apr 30 05:13 KI5_primary_unique.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Apr 30 05:13 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view KI5'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > KI5_HTSeq.out Ending samtools and htseq-count List files in scratch total 869M drwx------ 2 kkarri waxmanlab 4.0K Apr 30 05:25 . drwxrwxrwt. 52 root root 164K Apr 30 05:43 .. -rw-r--r-- 1 kkarri waxmanlab 231K Apr 30 05:46 KI5_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 306M Apr 30 05:13 KI5_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 483M Apr 30 05:25 KI5_sorted.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Apr 30 05:13 genes.gtf ========================================================== Finished on : Tue Apr 30 05:46:51 EDT 2019 0 hours, 38 minutes and 9 seconds elapsed. ==========================================================