----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: WT3 Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo Sample_Labels_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Apr 30 05:29:42 EDT 2019 Running on node : scc-kb2 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/CAR_Project_Data/GSE96650_LxRKo/Scripts/08a_Extract_Counts_HTSeq Current job ID : 5782293 Current job name : Step_08a_WT3 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/5782293.1.linga Loading required modules... List files in scratch directory: total 339M drwx------ 2 kkarri waxmanlab 4.0K Apr 30 05:41 . drwxrwxrwt. 83 root root 92K Apr 30 05:33 .. -rw-r--r-- 1 kkarri waxmanlab 0 Apr 30 05:41 WT3_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 259M Apr 30 05:35 WT3_primary_unique.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Apr 30 05:35 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view WT3'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > WT3_HTSeq.out Ending samtools and htseq-count List files in scratch total 748M drwx------ 2 kkarri waxmanlab 4.0K Apr 30 05:45 . drwxrwxrwt. 78 root root 92K Apr 30 06:00 .. -rw-r--r-- 1 kkarri waxmanlab 229K Apr 30 06:01 WT3_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 259M Apr 30 05:35 WT3_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 409M Apr 30 05:45 WT3_sorted.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Apr 30 05:35 genes.gtf ========================================================== Finished on : Tue Apr 30 06:01:43 EDT 2019 0 hours, 32 minutes and 1 seconds elapsed. ==========================================================