[bam_sort_core] merging from 5 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P KI6_sorted.bam || || || || Output file : KI6_assign_all_features.out || || Annotations : GeneSym_assign_all_features.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_all_features.gtf ... || || Features : 6105 || || Meta-features : 1713 || || Chromosomes/contigs : 25 || || || || Process BAM file KI6_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR5345549.3 73 chr12 105003916 50 41M * 0 0 N || || # NH:i:1 || || SRR5345549.4 83 chr6 121837489 50 43M = 121836604 - || || 928 N # NH:i:1 || || 493296 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 5297091 || || Successfully assigned fragments : 30890 (0.6%) || || Running time : 1.80 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================// ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P KI6_sorted.bam || || || || Output file : KI6_assign_only1_feature.out || || Annotations : GeneSym_assign_only1_feature.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_only1_feature.gtf ... || || Features : 315218 || || Meta-features : 22484 || || Chromosomes/contigs : 32 || || || || Process BAM file KI6_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR5345549.231 163 chr5 8949970 50 43M = 8950048 12 || || 0 N # NH:i:1 || || SRR5345549.232 99 chr5 89870307 50 15M266N28M = 89870 || || 698 2142 N # NH:i:1 || || 493296 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 5297091 || || Successfully assigned fragments : 4499615 (84.9%) || || Running time : 1.74 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//