[samopen] SAM header is present: 22 sequences. [bam_sort_core] merging from 4 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P WT5_sorted.bam || || || || Output file : WT5_featureCounts.out || || Annotations : intronic_only_gene_models_ncRNA_for_counting ... || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file intronic_only_gene_models_ncRNA_for_counting.gtf ... || || Features : 229209 || || Meta-features : 21891 || || Chromosomes/contigs : 32 || || || || Process BAM file WT5_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR5345536.1 99 chr19 38199035 50 43M = 38199135 25 || || 0 N # NH:i:1 || || SRR5345536.2 83 chr1 173156430 50 43M = 173156371 - || || 102 N # NH:i:1 || || 1414312 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 4554107 || || Successfully assigned fragments : 250022 (5.5%) || || Running time : 1.86 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//