[samopen] SAM header is present: 22 sequences. [bam_sort_core] merging from 4 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P WT6_sorted.bam || || || || Output file : WT6_featureCounts.out || || Annotations : intronic_only_gene_models_ncRNA_for_counting ... || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file intronic_only_gene_models_ncRNA_for_counting.gtf ... || || Features : 229209 || || Meta-features : 21891 || || Chromosomes/contigs : 32 || || || || Process BAM file WT6_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR5345537.2 147 chr17 57360541 50 43M = 57359599 - || || 985 N # NH:i:1 || || SRR5345537.4 83 chr11 69811264 50 43M = 69809549 -1 || || 758 N # NH:i:1 || || 1437093 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 4607748 || || Successfully assigned fragments : 251853 (5.5%) || || Running time : 1.57 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//