[bam_sort_core] merging from 4 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P WT9_sorted.bam || || || || Output file : WT9_assign_all_features.out || || Annotations : GeneSym_assign_all_features.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_all_features.gtf ... || || Features : 6105 || || Meta-features : 1713 || || Chromosomes/contigs : 25 || || || || Process BAM file WT9_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR5345540.6 153 chr5 122022846 50 24M562N18M * 0 0 || || N # NH:i:1 || || SRR5345540.8 153 chr1 121490909 50 41M * 0 0 N || || # NH:i:1 || || 492140 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 4444106 || || Successfully assigned fragments : 22822 (0.5%) || || Running time : 2.20 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================// ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P WT9_sorted.bam || || || || Output file : WT9_assign_only1_feature.out || || Annotations : GeneSym_assign_only1_feature.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_only1_feature.gtf ... || || Features : 315218 || || Meta-features : 22484 || || Chromosomes/contigs : 32 || || || || Process BAM file WT9_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || SRR5345540.241 147 chr18 46753026 50 43M = 46752932 || || -137 N # NH:i:1 || || SRR5345540.242 83 chr12 112353136 50 42M = 112353009 || || -169 N # NH:i:1 || || 492140 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 4444106 || || Successfully assigned fragments : 3790288 (85.3%) || || Running time : 1.94 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//