----------------------- Need shift 5 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/Dana/Scripts/08c_DiffExp_13_lncRNA_featureCounts Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/Dana Dataset_Label: GSE118757 ANNOTATION_FILE_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files ANNOTATION_FILE: /unprotected/projects/waxmanlab/routines/GTF_Files/ncRNA_output_filtered_final_gene.txt CONDITION_1_NAME: WTM_P14 CONDITION_2_NAME: WTF_P14 Lengths_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files/lengths GENE_LENGTHS_FILE: ncRNA_genebodies_for_counting_lengths.txt COUNT_DIR: LncRNA_GeneBody_GTF OUTPUT_PREFIX: DiffExp_v2_LncRNA_GeneBody DiffExp_Index: DiffExp_13e COL_SUFFIX: LncRNA_GeneBody COUNT_PROGRAM: featureCounts ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Mar 17 10:26:12 EDT 2020 Running on node : scc-tm3 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/Dana/Scripts/08c_DiffExp_13_lncRNA_featureCounts Current job ID : 3911195 Current job name : Step_08c_DiffExp_13e Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/3911195.1.linga Loading required modules... ------------------------------------------ Sample_DIR: WTM_1 Sample_ID: WTM_1 Description: WTM_1 M_Num: 1 Copy Condition_1 sample count files to Condition_1 folder Copy Condition_1 sample count summary files to Condition_1 folder Sample_DIR: WTM_2 Sample_ID: WTM_2 Description: WTM_2 M_Num: 2 Copy Condition_1 sample count files to Condition_1 folder Copy Condition_1 sample count summary files to Condition_1 folder Sample_DIR: WTM_3 Sample_ID: WTM_3 Description: WTM_3 M_Num: 3 Copy Condition_1 sample count files to Condition_1 folder Copy Condition_1 sample count summary files to Condition_1 folder M_Num_Cond1_List: 123 ------------------------------------------ ------------------------------------------ Sample_DIR: WTF_1 Sample_ID: WTF_1 Description: WTF_1 M_Num: 1 Copy Condition_2 sample count files to Condition_2 folder Copy Condition_2 sample count summary files to Condition_2 folder Sample_DIR: WTF_2 Sample_ID: WTF_2 Description: WTF_2 M_Num: 2 Copy Condition_2 sample count files to Condition_2 folder Copy Condition_2 sample count summary files to Condition_2 folder M_Num_Cond2_List: 12 ------------------------------------------ ========================================================== Number of replicates in each condition: NUM_REP_CONDITION1: 3 NUM_REP_CONDITION1: 3 ========================================================== Renaming input count files number of mapped reads (feature count summary) Status WTF_1_sorted.bam Assigned 15905345 Unassigned_Ambiguity 5184764 Unassigned_MultiMapping 0 Unassigned_NoFeatures 2786705 Unassigned_Unmapped 0 Unassigned_MappingQuality 0 Unassigned_FragmentLength 0 Unassigned_Chimera 0 Unassigned_Secondary 0 Unassigned_Nonjunction 0 Unassigned_Duplicate 0 Status WTF_2_sorted.bam Assigned 14745021 Unassigned_Ambiguity 4857337 Unassigned_MultiMapping 0 Unassigned_NoFeatures 2653695 Unassigned_Unmapped 0 Unassigned_MappingQuality 0 Unassigned_FragmentLength 0 Unassigned_Chimera 0 Unassigned_Secondary 0 Unassigned_Nonjunction 0 Unassigned_Duplicate 0 Status WTM_1_sorted.bam Assigned 10969942 Unassigned_Ambiguity 3386208 Unassigned_MultiMapping 0 Unassigned_NoFeatures 2651656 Unassigned_Unmapped 0 Unassigned_MappingQuality 0 Unassigned_FragmentLength 0 Unassigned_Chimera 0 Unassigned_Secondary 0 Unassigned_Nonjunction 0 Unassigned_Duplicate 0 Status WTM_2_sorted.bam Assigned 12022558 Unassigned_Ambiguity 3720199 Unassigned_MultiMapping 0 Unassigned_NoFeatures 3727845 Unassigned_Unmapped 0 Unassigned_MappingQuality 0 Unassigned_FragmentLength 0 Unassigned_Chimera 0 Unassigned_Secondary 0 Unassigned_Nonjunction 0 Unassigned_Duplicate 0 Status WTM_3_sorted.bam Assigned 14088350 Unassigned_Ambiguity 4169605 Unassigned_MultiMapping 0 Unassigned_NoFeatures 2569120 Unassigned_Unmapped 0 Unassigned_MappingQuality 0 Unassigned_FragmentLength 0 Unassigned_Chimera 0 Unassigned_Secondary 0 Unassigned_Nonjunction 0 Unassigned_Duplicate 0 ========================================================== List files in Input -rw-r--r-- 1 kkarri waxmanlab 713820 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P140.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P140.summary -rw-r--r-- 1 kkarri waxmanlab 713580 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P141.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P141.summary -rw-r--r-- 1 kkarri waxmanlab 711063 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P140.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P140.summary -rw-r--r-- 1 kkarri waxmanlab 711052 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P141.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P141.summary -rw-r--r-- 1 kkarri waxmanlab 713838 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P142.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P142.summary -rw-r--r-- 1 kkarri waxmanlab 815529 Mar 17 10:26 /scratch/3911195.1.linga/Input/ncRNA_genebodies_for_counting_lengths.txt /scratch/3911195.1.linga/Input/WTF_P14: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Mar 17 10:26 . drwxr-xr-x 4 kkarri waxmanlab 4096 Mar 17 10:26 .. /scratch/3911195.1.linga/Input/WTM_P14: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Mar 17 10:26 . drwxr-xr-x 4 kkarri waxmanlab 4096 Mar 17 10:26 .. ========================================================== Starting to run my commands Printing Rscript command: Rscript differentialAnalysis.R WTM_P14 WTF_P14 3 2 /unprotected/projects/waxmanlab/routines/GTF_Files/ncRNA_output_filtered_final_gene.txt /scratch/3911195.1.linga/Input DiffExp_v2_LncRNA_GeneBody ncRNA_genebodies_for_counting_lengths.txt [1] "Arguments for differentialAnalysisDESeq.R:" [1] "WTM_P14" [1] "WTF_P14" [1] 3 [1] 2 [1] "/unprotected/projects/waxmanlab/routines/GTF_Files/ncRNA_output_filtered_final_gene.txt" [1] "/scratch/3911195.1.linga/Input" [1] "DiffExp_v2_LncRNA_GeneBody" [1] "ncRNA_genebodies_for_counting_lengths.txt" [1] "sum1:67091.200960162" "sum1:67493.02039777" "sum1:64441.6533555699" [4] "sum1:66341.958237834" [1] "sum2:66854.334889291" "sum2:65198.5268871595" "sum2:66026.4308882253" [1] 15558 23 [1] 15558 59 [1] "chr1" "chr2" "chr3" "chr4" "chr5" "chr6" "chr7" "chr8" "chr9" [1] "chr10" "chr11" "chr12" "chr13" "chr14" "chr15" "chr16" "chr17" "chr18" [10] "chr19" [1] "chrX" "chrY" [1] "output file is in: /scratch/3911195.1.linga/Input/DiffExp_v2_LncRNA_GeneBody_WTM_P14_WTF_P14.txt" ========================================================== Create SEGEX formatted file: Printing Rscript command: Rscript formatForSegex_ver3.R DiffExp_v2_LncRNA_GeneBody_WTM_P14_WTF_P14.txt WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload 1 LncRNA_GeneBody Create SEGEX formatted file: Printing Rscript command: Rscript formatForSegex_ver4.R DiffExp_v2_LncRNA_GeneBody_WTM_P14_WTF_P14.txt WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload 1 LncRNA_GeneBody ========================================================== Comparison_Info: WTF_P14_GSE118757_12_WTM_P14_GSE118757_123 #---------------------------------------------------------------------------------- Running Diff_Genes.R #---------------------------------------------------------------------------------- Printing Rscript command: Rscript Diff_Genes.R WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_DESeq.txt 2 0.05 DESeq LncRNA_GeneBody'_'WTF_P14_GSE118757_12_WTM_P14_GSE118757_123 [1] "Print arguments:" [1] "-----------------" [1] "Differential expression_Output:" [1] "WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_DESeq.txt" [1] "fold_change_cutoff:" [1] "2" [1] "padj_cutoff:" [1] "0.05" [1] "postfix:" [1] "DESeq" [1] "count_method:" [1] "LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123" [1] "-----------------" [1] "Differential Gene Counts" [1] "Number of significant differential genes with positive fold change (Up Genes):" [1] 0 7 [1] "Number of significant differential genes with negative fold change (Down Genes):" [1] 0 7 [1] "Check out Up_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt!" [1] "Check out Down_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt!" Rscript Diff_Genes.R WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_EdgeR.txt 2 0.05 EdgeR LncRNA_GeneBody'_'WTF_P14_GSE118757_12_WTM_P14_GSE118757_123 [1] "Print arguments:" [1] "-----------------" [1] "Differential expression_Output:" [1] "WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_EdgeR.txt" [1] "fold_change_cutoff:" [1] "2" [1] "padj_cutoff:" [1] "0.05" [1] "postfix:" [1] "EdgeR" [1] "count_method:" [1] "LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123" [1] "-----------------" [1] "Differential Gene Counts" [1] "Number of significant differential genes with positive fold change (Up Genes):" [1] 0 7 [1] "Number of significant differential genes with negative fold change (Down Genes):" [1] 0 7 [1] "Check out Up_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt!" [1] "Check out Down_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt!" #---------------------------------------------------------------------------------- Running Venn_Diff_Genes.R #---------------------------------------------------------------------------------- Printing Rscript command: #---------------------------------------------------------------------------------- Rscript Venn_Diff_Genes.R Down_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt Down_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt LncRNA_GeneBody_Counting DiffExp_13e [1] "Print arguments:" [1] "-----------------" [1] "File1:" [1] "Down_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt" [1] "File2:" [1] "Down_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt" [1] "Subtitle:" [1] "LncRNA_GeneBody_Counting" [1] "DiffExp_Index:" [1] "DiffExp_13e" [1] "-----------------" [1] "Down.DESeq.GeneBody.HTSeq" [1] "Down.EdgeR.GeneBody.HTSeq" #---------------------------------------------------------------------------------- Rscript Venn_Diff_Genes.R Up_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt Up_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt LncRNA_GeneBody_Counting DiffExp_13e [1] "Print arguments:" [1] "-----------------" [1] "File1:" [1] "Up_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt" [1] "File2:" [1] "Up_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt" [1] "Subtitle:" [1] "LncRNA_GeneBody_Counting" [1] "DiffExp_Index:" [1] "DiffExp_13e" [1] "-----------------" [1] "Up.DESeq.GeneBody.HTSeq" [1] "Up.EdgeR.GeneBody.HTSeq" #---------------------------------------------------------------------------------- #---------------------------------------------------------------------------------- Merging Count.Table(s) into one text file #---------------------------------------------------------------------------------- Comparison_Info: WTF_P14_GSE118757_12_WTM_P14_GSE118757_123 #---------------------------------------------------------------------------------- ========================================================== Renaming the Differential_Expression_File ========================================================== List files in Input -rw-r--r-- 1 kkarri waxmanlab 0 Mar 17 10:27 /scratch/3911195.1.linga/Input/DiffExp_13e_Venn_Tables_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt -rw-r--r-- 1 kkarri waxmanlab 4845651 Mar 17 10:26 /scratch/3911195.1.linga/Input/DiffExp_v2_LncRNA_GeneBody_WTM_P14_WTF_P14.txt -rwxr-xr-x 1 kkarri waxmanlab 7706 Mar 17 10:26 /scratch/3911195.1.linga/Input/Diff_Genes.R -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:26 /scratch/3911195.1.linga/Input/Down_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:26 /scratch/3911195.1.linga/Input/Down_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:26 /scratch/3911195.1.linga/Input/Up_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:26 /scratch/3911195.1.linga/Input/Up_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123.txt -rw-r--r-- 1 kkarri waxmanlab 4408 Mar 17 10:27 /scratch/3911195.1.linga/Input/VennDiagram2020-03-17_10-26-59.log -rwxr-xr-x 1 kkarri waxmanlab 9993 Mar 17 10:26 /scratch/3911195.1.linga/Input/Venn_Diff_Genes.R -rw-r--r-- 1 kkarri waxmanlab 713820 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P140.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P140.summary -rw-r--r-- 1 kkarri waxmanlab 713580 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P141.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P141.summary -rw-r--r-- 1 kkarri waxmanlab 1607977 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_DESeq.txt -rw-r--r-- 1 kkarri waxmanlab 1654651 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_EdgeR.txt -rw-r--r-- 1 kkarri waxmanlab 779828 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_TPM_DESeq.txt -rw-r--r-- 1 kkarri waxmanlab 934472 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_TPM_EdgeR.txt -rw-r--r-- 1 kkarri waxmanlab 711063 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P140.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P140.summary -rw-r--r-- 1 kkarri waxmanlab 711052 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P141.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P141.summary -rw-r--r-- 1 kkarri waxmanlab 713838 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P142.out -rw-r--r-- 1 kkarri waxmanlab 297 Mar 17 10:26 /scratch/3911195.1.linga/Input/WTM_P142.summary -rwxr-xr-x 1 kkarri waxmanlab 4968 Mar 17 10:26 /scratch/3911195.1.linga/Input/formatForSegex_ver3.R -rwxr-xr-x 1 kkarri waxmanlab 4972 Mar 17 10:26 /scratch/3911195.1.linga/Input/formatForSegex_ver4.R -rw-r--r-- 1 kkarri waxmanlab 815529 Mar 17 10:26 /scratch/3911195.1.linga/Input/ncRNA_genebodies_for_counting_lengths.txt /scratch/3911195.1.linga/Input/WTF_P14: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Mar 17 10:26 . drwxr-xr-x 4 kkarri waxmanlab 4096 Mar 17 10:27 .. /scratch/3911195.1.linga/Input/WTM_P14: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Mar 17 10:26 . drwxr-xr-x 4 kkarri waxmanlab 4096 Mar 17 10:27 .. ========================================================== List files in scratch total 3.4M drwx------ 3 kkarri waxmanlab 4.0K Mar 17 10:27 . drwxrwxrwt. 10 root root 4.0K Mar 17 10:26 .. -rw-r--r-- 1 kkarri waxmanlab 87 Mar 17 10:26 Condition_1.txt -rw-r--r-- 1 kkarri waxmanlab 69 Mar 17 10:26 Condition_2.txt drwxr-xr-x 4 kkarri waxmanlab 4.0K Mar 17 10:27 Input -rwxr-xr-x 1 kkarri waxmanlab 15K Mar 17 10:26 differentialAnalysis.R -rw-r--r-- 1 kkarri waxmanlab 3.3M Mar 17 10:26 ncRNA_output_filtered_final_gene.txt ========================================================== Re-naming files in OUTPUT_DIR Need to append the COUNT_PROGRAM name to all output files List files in OUTPUT_DIR total 23M drwxr-xr-x 2 kkarri waxmanlab 4.0K Mar 17 10:19 . drwxr-xr-x 7 kkarri waxmanlab 4.0K Mar 17 10:18 .. -rw-r--r-- 1 kkarri waxmanlab 0 Mar 17 10:19 DiffExp_13e_Venn_Tables_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 4.7M Mar 17 10:19 DiffExp_v2_LncRNA_GeneBody_WTM_P14_WTF_P14_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:19 Down_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:19 Down_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:19 Up_Genes_DESeq_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 188 Mar 17 10:19 Up_Genes_EdgeR_LncRNA_GeneBody_WTF_P14_GSE118757_12_WTM_P14_GSE118757_123_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 1.6M Mar 17 10:19 WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_DESeq_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 1.6M Mar 17 10:19 WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_EdgeR_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 762K Mar 17 10:19 WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_TPM_DESeq_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 913K Mar 17 10:19 WTF_P14_GSE118757_12_vs_WTM_P14_GSE118757_123_DiffExp_v2_LncRNA_GeneBody_forSEGEXUpload_TPM_EdgeR_featureCounts.txt ========================================================== ========================================================== Finished on : Tue Mar 17 10:27:03 EDT 2020 0 minutes and 51 seconds elapsed. ==========================================================