----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: G123_M1 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Dec 18 18:53:48 EST 2018 Running on node : scc-kb5 Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08a_Extract_Counts_HTSeq Current job ID : 365154 Current job name : Step_08a_G123_M1 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/365154.1.linga Loading required modules... List files in scratch directory: total 1.4G drwx------ 2 kkarri waxmanlab 4.0K Dec 18 18:54 . drwxrwxrwt. 40 root root 268K Dec 18 18:53 .. -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 18:54 G123_M1_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.3G Dec 18 18:54 G123_M1_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 72M Dec 18 18:54 RefSeq_GeneBody.gtf Starting to run my commands Starting samtools and htseq-count samtools view G123_M1'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - RefSeq_GeneBody.gtf > G123_M1_HTSeq.out Ending samtools and htseq-count List files in scratch total 3.2G drwx------ 2 kkarri waxmanlab 4.0K Dec 18 19:15 . drwxrwxrwt. 24 root root 268K Dec 18 20:12 .. -rw-r--r-- 1 kkarri waxmanlab 244K Dec 18 20:13 G123_M1_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.3G Dec 18 18:54 G123_M1_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 1.8G Dec 18 19:15 G123_M1_sorted.bam -rw-r--r-- 1 kkarri waxmanlab 72M Dec 18 18:54 RefSeq_GeneBody.gtf ========================================================== Finished on : Tue Dec 18 20:13:28 EST 2018 1 hours, 19 minutes and 40 seconds elapsed. ==========================================================