-----------------------
Need shift 1 command (have more than 9 arguments):
-----------------------
-----------------------
Start of variable list:
-----------------------
Sample_ID:
G123_M12
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/G123_Env
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
MODE:
intersection-nonempty
STRANDEDNESS_HTSeq:
reverse
FEATURE_ID:
gene_id
SCRIPT_DIR:
/restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08a_Extract_Counts_HTSeq
ANNOTATION_FILE:
Intron_Only_Regions.gtf
FEATURE_TYPE:
Intronic_Only
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Tue Dec 18 18:54:01 EST 2018
Running on node : scc-ka5
Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08a_Extract_Counts_HTSeq
Current job ID : 365200
Current job name : Step_08a_G123_M12
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/365200.1.linga

Loading required modules...


List files in scratch directory:

total 1.3G
drwx------   2 kkarri waxmanlab 4.0K Dec 18 18:59 .
drwxrwxrwt. 30 root   root      128K Dec 18 18:53 ..
-rw-r--r--   1 kkarri waxmanlab    0 Dec 18 18:59 G123_M12_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 1.3G Dec 18 18:54 G123_M12_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab  18M Dec 18 18:55 Intron_Only_Regions.gtf

Starting to run my commands


Starting samtools and htseq-count


Starting splice read filter


Ending splice read filter

samtools view G123_M12'_sorted'.bam | htseq-count --stranded=reverse --type=Intronic_Only --idattr=gene_id --order=name --mode=intersection-nonempty - Intron_Only_Regions.gtf  > G123_M12_HTSeq.out

Ending samtools and htseq-count


List files in scratch

total 28G
drwx------   2 kkarri waxmanlab 4.0K Dec 18 20:19 .
drwxrwxrwt. 26 root   root      128K Dec 18 20:18 ..
-rw-r--r--   1 kkarri waxmanlab 241K Dec 18 20:19 G123_M12_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 1.2G Dec 18 19:10 G123_M12_primary_no_splice.bam
-rw-r--r--   1 kkarri waxmanlab 1.3G Dec 18 18:54 G123_M12_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 1.7G Dec 18 19:27 G123_M12_sorted.bam
-rw-r--r--   1 kkarri waxmanlab   99 Dec 18 20:19 HTSeq_Extra_Lines.txt
-rw-r--r--   1 kkarri waxmanlab  951 Dec 18 19:00 Header.txt
-rw-r--r--   1 kkarri waxmanlab  18M Dec 18 18:55 Intron_Only_Regions.gtf
-rwxr-xr-x   1 kkarri waxmanlab  38K Dec 18 20:19 RefSeq_Genes_Zero_Count.txt
-rw-r--r--   1 kkarri waxmanlab 8.2G Dec 18 19:02 temp1.sam
-rw-r--r--   1 kkarri waxmanlab 7.5G Dec 18 19:03 temp2.sam
-rw-r--r--   1 kkarri waxmanlab 7.5G Dec 18 19:03 temp3.sam
==========================================================
Finished on : Tue Dec 18 20:19:44 EST 2018
1 hours, 25 minutes and 43 seconds elapsed.
==========================================================