----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: G123_M8 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Dec 18 18:53:46 EST 2018 Running on node : scc-tl4 Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08a_Extract_Counts_HTSeq Current job ID : 365183 Current job name : Step_08a_G123_M8 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/365183.1.linga Loading required modules... List files in scratch directory: total 1.4G drwx------ 2 kkarri waxmanlab 4.0K Dec 18 18:54 . drwxrwxrwt. 12 root root 40K Dec 18 18:53 .. -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 18:54 G123_M8_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.3G Dec 18 18:53 G123_M8_primary_unique.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Dec 18 18:54 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view G123_M8'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > G123_M8_HTSeq.out Ending samtools and htseq-count List files in scratch total 3.1G drwx------ 2 kkarri waxmanlab 4.0K Dec 18 19:05 . drwxrwxrwt. 12 root root 40K Dec 18 19:11 .. -rw-r--r-- 1 kkarri waxmanlab 239K Dec 18 19:29 G123_M8_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 1.3G Dec 18 18:53 G123_M8_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 1.8G Dec 18 19:05 G123_M8_sorted.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Dec 18 18:54 genes.gtf ========================================================== Finished on : Tue Dec 18 19:29:50 EST 2018 0 hours, 36 minutes and 4 seconds elapsed. ==========================================================