----------------------- Start of variable list: ----------------------- Sample_ID: G123_M4 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files STRANDEDNESS_featureCount: 2 FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08b_Extract_Counts_featureCounts ANNOTATION_FILE: Exon_Only_Regions.gtf FEATURE_TYPE: Exonic_Only ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Dec 18 21:15:08 EST 2018 Running on node : scc-kb2 Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08b_Extract_Counts_featureCounts Current job ID : 366093 Current job name : Step_08b_G123_M4 Task index number : undefined Parameter for multiple cores : 16 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/366093.1.linga Loading required modules... List files in scratch directory: total 1.9G drwx------ 2 kkarri waxmanlab 4.0K Dec 18 21:15 . drwxrwxrwt. 45 root root 92K Dec 18 21:15 .. -rw-r--r-- 1 kkarri waxmanlab 19M Dec 18 21:15 Exon_Only_Regions.gtf -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:15 G123_M4_featureCounts.out -rw-r--r-- 1 kkarri waxmanlab 1.8G Dec 18 21:15 G123_M4_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 18M Dec 18 21:15 Intron_Only_Regions.gtf Starting to run my commands Starting featureCounts Starting Intron_Only_Regions read overlap filter Ending Intron_Only_Regions read overlap filter GTF file name: Exon_Only_Regions Rscript Parse_GTF.R Exon_Only_Regions.gtf GeneSym_List.txt WARNING: ignoring environment value of R_HOME Running assign_all_features counting featureCounts -O -p -T 16 -s 2 -g gene_id -t Exonic_Only -a GeneSym_assign_all_features.gtf -o G123_M4_assign_all_features.out G123_M4'_sorted'.bam Running assign_only1_feature counting featureCounts -p -T 16 -s 2 -g gene_id -t Exonic_Only -a GeneSym_assign_only1_feature.gtf -o G123_M4_assign_only1_feature.out G123_M4'_sorted'.bam Processing *_assign_*.out Done processing file. Ending featureCounts List files in scratch total 1.9G -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:15 *_assign_*.out drwx------ 2 kkarri waxmanlab 4.0K Dec 18 21:15 . drwxrwxrwt. 45 root root 92K Dec 18 21:15 .. -rw-r--r-- 1 kkarri waxmanlab 19M Dec 18 21:15 Exon_Only_Regions.gtf -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:15 G123_M4_featureCounts.out -rw-r--r-- 1 kkarri waxmanlab 65 Dec 18 21:15 G123_M4_featureCounts.out.summary -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:15 G123_M4_primary_no_Intron_Only.bam -rw-r--r-- 1 kkarri waxmanlab 1.8G Dec 18 21:15 G123_M4_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 14K Dec 18 21:15 GeneSym_List.txt -rw-r--r-- 1 kkarri waxmanlab 18M Dec 18 21:15 Intron_Only_Regions.gtf -rwxr-xr-x 1 kkarri waxmanlab 6.8K Dec 18 21:15 Parse_GTF.R ========================================================== Finished on : Tue Dec 18 21:15:38 EST 2018 0 hours, 0 minutes and 30 seconds elapsed. ==========================================================