----------------------- Start of variable list: ----------------------- Sample_ID: G123_M5 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files STRANDEDNESS_featureCount: 2 FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08b_Extract_Counts_featureCounts ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Dec 18 21:25:06 EST 2018 Running on node : scc-kb2 Current directory : /restricted/projectnb/waxmanlab/kkarri/G123_Env/Scripts/08b_Extract_Counts_featureCounts Current job ID : 366103 Current job name : Step_08b_G123_M5 Task index number : undefined Parameter for multiple cores : 16 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/366103.1.linga Loading required modules... List files in scratch directory: total 1.3G drwx------ 2 kkarri waxmanlab 4.0K Dec 18 21:25 . drwxrwxrwt. 46 root root 92K Dec 18 21:25 .. -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:25 G123_M5_featureCounts.out -rw-r--r-- 1 kkarri waxmanlab 1.2G Dec 18 21:25 G123_M5_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 72M Dec 18 21:25 RefSeq_GeneBody.gtf Starting to run my commands Starting featureCounts GTF file name: RefSeq_GeneBody Rscript Parse_GTF.R RefSeq_GeneBody.gtf GeneSym_List.txt WARNING: ignoring environment value of R_HOME Running assign_all_features counting featureCounts -O -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_all_features.gtf -o G123_M5_assign_all_features.out G123_M5'_sorted'.bam Running assign_only1_feature counting featureCounts -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_only1_feature.gtf -o G123_M5_assign_only1_feature.out G123_M5'_sorted'.bam Processing *_assign_*.out Done processing file. Ending featureCounts List files in scratch total 2.9G -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:45 *_assign_*.out drwx------ 2 kkarri waxmanlab 4.0K Dec 18 21:45 . drwxrwxrwt. 46 root root 92K Dec 18 21:40 .. -rw-r--r-- 1 kkarri waxmanlab 0 Dec 18 21:45 G123_M5_featureCounts.out -rw-r--r-- 1 kkarri waxmanlab 65 Dec 18 21:45 G123_M5_featureCounts.out.summary -rw-r--r-- 1 kkarri waxmanlab 1.2G Dec 18 21:25 G123_M5_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 1.6G Dec 18 21:45 G123_M5_sorted.bam -rw-r--r-- 1 kkarri waxmanlab 14K Dec 18 21:45 GeneSym_List.txt -rwxr-xr-x 1 kkarri waxmanlab 6.8K Dec 18 21:45 Parse_GTF.R -rw-r--r-- 1 kkarri waxmanlab 72M Dec 18 21:25 RefSeq_GeneBody.gtf ========================================================== Finished on : Tue Dec 18 21:45:13 EST 2018 0 hours, 20 minutes and 7 seconds elapsed. ==========================================================