----------------------- Start of variable list: ----------------------- Sample_ID: G160_M22 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/G160_Samples Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/G160_Samples/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files STRANDEDNESS_featureCount: 2 FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/G160_Samples/Scripts/08b_Extract_Counts_featureCounts ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Wed Aug 30 21:50:33 EDT 2017 Running on node : scc-kb6 Current directory : /restricted/projectnb/waxmanlab/kkarri/G160_Samples/Scripts/08b_Extract_Counts_featureCounts Current job ID : 474026 Current job name : Step_08b_G160_M22 Task index number : undefined Parameter for multiple cores : 16 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/474026.1.linga Loading required modules... List files in scratch directory: total 1.9G drwxr-xr-x 2 kkarri pulmseq 4.0K Aug 30 21:50 . drwxrwxrwt. 65 root root 12K Aug 30 21:50 .. -rw-r--r-- 1 kkarri pulmseq 0 Aug 30 21:50 G160_M22_featureCounts.out -rw-r--r-- 1 kkarri pulmseq 1.8G Aug 30 21:50 G160_M22_primary_unique.bam -rw-r--r-- 1 kkarri pulmseq 72M Aug 30 21:50 RefSeq_GeneBody.gtf Starting to run my commands Starting featureCounts GTF file name: RefSeq_GeneBody Rscript Parse_GTF.R RefSeq_GeneBody.gtf GeneSym_List.txt [1] "Print arguments:" [1] "-----------------" [1] "GTF_File:" [1] "RefSeq_GeneBody.gtf" [1] "Subset_List:" [1] "GeneSym_List.txt" [1] "-----------------" [1] "Loading RefSeq_GeneBody.gtf" [1] "Loading GeneSym_List.txt" [1] "Number of gene symbols in common:" [1] "1713" [1] "Percentage overlap:" [1] "98%" [1] "How many GeneSym in the GTF list and not in Subset list:" [1] "(Expect high number)" [1] 22484 [1] "How many GeneSym in the Subset list and not in GTF list:" [1] "(Due to updated RefSeq gene annotations)" [1] 27 [1] "Check out GeneSym_assign_all_features.gtf!" [1] "GeneSym_assign_only1_feature.gtf!" Running assign_all_features counting featureCounts -O -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_all_features.gtf -o G160_M22_assign_all_features.out G160_M22'_sorted'.bam Running assign_only1_feature counting featureCounts -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_only1_feature.gtf -o G160_M22_assign_only1_feature.out G160_M22'_sorted'.bam Processing G160_M22_assign_all_features.out Done processing file. Processing G160_M22_assign_only1_feature.out Done processing file. Ending featureCounts List files in scratch total 4.3G drwxr-xr-x 2 kkarri pulmseq 20K Aug 30 22:42 . drwxrwxrwt. 67 root root 12K Aug 30 22:10 .. -rw-r--r-- 1 kkarri pulmseq 17K Aug 30 22:42 G160_M22_assign_all_features.out -rw-r--r-- 1 kkarri pulmseq 292 Aug 30 22:31 G160_M22_assign_all_features.out.summary -rw-r--r-- 1 kkarri pulmseq 225K Aug 30 22:42 G160_M22_assign_only1_feature.out -rw-r--r-- 1 kkarri pulmseq 298 Aug 30 22:42 G160_M22_assign_only1_feature.out.summary -rw-r--r-- 1 kkarri pulmseq 242K Aug 30 22:42 G160_M22_featureCounts.out -rw-r--r-- 1 kkarri pulmseq 657 Aug 30 22:42 G160_M22_featureCounts.out.summary -rw-r--r-- 1 kkarri pulmseq 1.8G Aug 30 21:50 G160_M22_primary_unique.bam -rw-r--r-- 1 kkarri pulmseq 2.4G Aug 30 22:20 G160_M22_sorted.bam -rw-r--r-- 1 kkarri pulmseq 14K Aug 30 22:20 GeneSym_List.txt -rw-r--r-- 1 kkarri pulmseq 1.3M Aug 30 22:20 GeneSym_assign_all_features.gtf -rw-r--r-- 1 kkarri pulmseq 67M Aug 30 22:21 GeneSym_assign_only1_feature.gtf -rwxr-xr-x 1 kkarri pulmseq 6.8K Aug 30 22:20 Parse_GTF.R -rw-r--r-- 1 kkarri pulmseq 72M Aug 30 21:50 RefSeq_GeneBody.gtf ========================================================== Finished on : Wed Aug 30 22:42:01 EDT 2017 0 hours, 51 minutes and 28 seconds elapsed. ==========================================================