Loading required package: S4Vectors Loading required package: stats4 Loading required package: BiocGenerics Loading required package: parallel Attaching package: 'BiocGenerics' The following objects are masked from 'package:parallel': clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport, clusterMap, parApply, parCapply, parLapply, parLapplyLB, parRapply, parSapply, parSapplyLB The following objects are masked from 'package:stats': IQR, mad, sd, var, xtabs The following objects are masked from 'package:base': Filter, Find, Map, Position, Reduce, anyDuplicated, append, as.data.frame, basename, cbind, colnames, dirname, do.call, duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted, lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table, tapply, union, unique, unsplit, which, which.max, which.min Attaching package: 'S4Vectors' The following object is masked from 'package:base': expand.grid Loading required package: IRanges Loading required package: GenomicRanges Loading required package: GenomeInfoDb Loading required package: SummarizedExperiment Loading required package: Biobase Welcome to Bioconductor Vignettes contain introductory material; view with 'browseVignettes()'. To cite Bioconductor, see 'citation("Biobase")', and for packages 'citation("pkgname")'. Loading required package: DelayedArray Loading required package: matrixStats Attaching package: 'matrixStats' The following objects are masked from 'package:Biobase': anyMissing, rowMedians Loading required package: BiocParallel Attaching package: 'DelayedArray' The following objects are masked from 'package:matrixStats': colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges The following objects are masked from 'package:base': aperm, apply, rowsum Registered S3 methods overwritten by 'ggplot2': method from [.quosures rlang c.quosures rlang print.quosures rlang Loading required package: limma Attaching package: 'limma' The following object is masked from 'package:DESeq2': plotMA The following object is masked from 'package:BiocGenerics': plotMA converting counts to integer mode estimating size factors estimating dispersions Error in checkForExperimentalReplicates(object, modelMatrix) : The design matrix has the same number of samples and coefficients to fit, so estimation of dispersion is not possible. Treating samples as replicates was deprecated in v1.20 and no longer supported since v1.22. Execution halted Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") : cannot open file 'DiffExp_v2_GeneBody_F_Soluble_Nuclear_F_Chromatin_Pellet.txt': No such file or directory Execution halted Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") : cannot open file 'DiffExp_v2_GeneBody_F_Soluble_Nuclear_F_Chromatin_Pellet.txt': No such file or directory Execution halted Loading required package: grid Loading required package: futile.logger Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") : cannot open file 'Down_Genes_DESeq_GeneBody_F_Chromatin_Pellet_G160_M4_F_Soluble_Nuclear_G160_M3.txt': No such file or directory Execution halted Loading required package: grid Loading required package: futile.logger Error in file(file, "rt") : cannot open the connection Calls: read.table -> file In addition: Warning message: In file(file, "rt") : cannot open file 'Up_Genes_DESeq_GeneBody_F_Chromatin_Pellet_G160_M4_F_Soluble_Nuclear_G160_M3.txt': No such file or directory Execution halted cat: Count.Table.*.txt: No such file or directory rm: cannot remove 'Count.Table.*.txt': No such file or directory cp: cannot stat '/scratch/8778112.1.linga/Input/DiffExp_v2_GeneBody_F_Soluble_Nuclear_F_Chromatin_Pellet.txt': No such file or directory cp: cannot stat '/scratch/8778112.1.linga/Input/F_Chromatin_Pellet_G160_M4_vs_F_Soluble_Nuclear_G160_M3_DiffExp_v2_GeneBody_forSEGEXUpload*': No such file or directory cp: cannot stat '/scratch/8778112.1.linga/Input/*_Genes*.txt': No such file or directory cp: cannot stat '/scratch/8778112.1.linga/Input/*.png': No such file or directory mv: cannot stat '*.png': No such file or directory