----------------------- Start of variable list: ----------------------- Sample_ID: G166_M11 Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV Sample_Labels_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files STRANDEDNESS_featureCount: 2 FEATURE_ID: gene_id SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts ANNOTATION_FILE: exonic_only_gene_models_ncRNA_for_counting.gtf FEATURE_TYPE: Exonic_Only ----------------------- End of variable list ----------------------- ========================================================== Starting on : Tue Apr 23 12:48:09 EDT 2019 Running on node : scc-pg7 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts Current job ID : 5542385 Current job name : Step_08b_G166_M11 Task index number : undefined Parameter for multiple cores : 16 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/5542385.1.p16 Loading required modules... List files in scratch directory: total 777M drwx------ 2 kkarri waxmanlab 4.0K Apr 23 12:48 . drwxrwxrwt. 26 root root 364K Apr 23 12:48 .. -rw-r--r-- 1 kkarri waxmanlab 0 Apr 23 12:48 G166_M11_featureCounts.out -rw-r--r-- 1 kkarri waxmanlab 742M Apr 23 12:48 G166_M11_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 19M Apr 23 12:48 Intron_Only_Regions.gtf -rw-r--r-- 1 kkarri waxmanlab 17M Apr 23 12:48 exonic_only_gene_models_ncRNA_for_counting.gtf Starting to run my commands Starting featureCounts Starting Intron_Only_Regions read overlap filter Ending Intron_Only_Regions read overlap filter featureCounts -p -T 16 -s 2 -g gene_id -t Exonic_Only -a exonic_only_gene_models_ncRNA_for_counting.gtf -o G166_M11_featureCounts.out G166_M11'_sorted'.bam Ending featureCounts List files in scratch total 1.6G drwx------ 2 kkarri waxmanlab 20K Apr 23 12:55 . drwxrwxrwt. 26 root root 364K Apr 23 12:48 .. -rw-r--r-- 1 kkarri waxmanlab 694K Apr 23 12:55 G166_M11_featureCounts.out -rw-r--r-- 1 kkarri waxmanlab 297 Apr 23 12:55 G166_M11_featureCounts.out.summary -rw-r--r-- 1 kkarri waxmanlab 278M Apr 23 12:50 G166_M11_primary_no_Intron_Only.bam -rw-r--r-- 1 kkarri waxmanlab 742M Apr 23 12:48 G166_M11_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 488M Apr 23 12:53 G166_M11_sorted.bam -rw-r--r-- 1 kkarri waxmanlab 19M Apr 23 12:48 Intron_Only_Regions.gtf -rw-r--r-- 1 kkarri waxmanlab 17M Apr 23 12:48 exonic_only_gene_models_ncRNA_for_counting.gtf ========================================================== Finished on : Tue Apr 23 12:55:22 EDT 2019 0 hours, 7 minutes and 13 seconds elapsed. ==========================================================