-----------------------
Start of variable list:
-----------------------
Sample_ID:
G166_M12
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files
STRANDEDNESS_featureCount:
2
FEATURE_ID:
gene_id
SCRIPT_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts
ANNOTATION_FILE:
genes.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Tue Apr 23 12:57:23 EDT 2019
Running on node : scc-kb8
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts
Current job ID : 5542394
Current job name : Step_08b_G166_M12
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/5542394.1.linga

Loading required modules...


List files in scratch directory:

total 866M
drwx------  2 kkarri waxmanlab 4.0K Apr 23 12:57 .
drwxrwxrwt. 7 root   root      112K Apr 23 12:57 ..
-rw-r--r--  1 kkarri waxmanlab    0 Apr 23 12:57 G166_M12_featureCounts.out
-rw-r--r--  1 kkarri waxmanlab 786M Apr 23 12:57 G166_M12_primary_unique.bam
-rwxr-xr-x  1 kkarri waxmanlab  81M Apr 23 12:57 genes.gtf

Starting to run my commands


Starting featureCounts

featureCounts -p  -T 16 -s 2 -g gene_id -t exon -a genes.gtf -o G166_M12_featureCounts.out G166_M12'_sorted'.bam

Ending featureCounts


List files in scratch

total 2.2G
drwx------  2 kkarri waxmanlab  20K Apr 23 13:16 .
drwxrwxrwt. 7 root   root      112K Apr 23 13:15 ..
-rw-r--r--  1 kkarri waxmanlab 234K Apr 23 13:16 G166_M12_featureCounts.out
-rw-r--r--  1 kkarri waxmanlab  298 Apr 23 13:16 G166_M12_featureCounts.out.summary
-rw-r--r--  1 kkarri waxmanlab 786M Apr 23 12:57 G166_M12_primary_unique.bam
-rw-r--r--  1 kkarri waxmanlab 1.3G Apr 23 13:10 G166_M12_sorted.bam
-rwxr-xr-x  1 kkarri waxmanlab  81M Apr 23 12:57 genes.gtf
==========================================================
Finished on : Tue Apr 23 13:16:21 EDT 2019
0 hours, 18 minutes and 58 seconds elapsed.
==========================================================