-----------------------
Start of variable list:
-----------------------
Sample_ID:
G166_M5
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files
STRANDEDNESS_featureCount:
2
FEATURE_ID:
gene_id
SCRIPT_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts
ANNOTATION_FILE:
ncRNA_exon_for_counting.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Tue Apr 23 11:36:41 EDT 2019
Running on node : scc-ka5
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts
Current job ID : 5542340
Current job name : Step_08b_G166_M5
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/5542340.1.linga

Loading required modules...


List files in scratch directory:

total 1.2G
drwx------   2 kkarri waxmanlab 4.0K Apr 23 11:37 .
drwxrwxrwt. 78 root   root      128K Apr 23 11:36 ..
-rw-r--r--   1 kkarri waxmanlab    0 Apr 23 11:37 G166_M5_featureCounts.out
-rw-r--r--   1 kkarri waxmanlab 882M Apr 23 11:37 G166_M5_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 254M Apr 23 11:37 ncRNA_exon_for_counting.gtf

Starting to run my commands


Starting featureCounts

featureCounts -p  -T 16 -s 2 -g gene_id -t exon -a ncRNA_exon_for_counting.gtf -o G166_M5_featureCounts.out G166_M5'_sorted'.bam

Ending featureCounts


List files in scratch

total 2.7G
drwx------   2 kkarri waxmanlab  20K Apr 23 12:01 .
drwxrwxrwt. 75 root   root      128K Apr 23 11:53 ..
-rw-r--r--   1 kkarri waxmanlab 702K Apr 23 12:01 G166_M5_featureCounts.out
-rw-r--r--   1 kkarri waxmanlab  298 Apr 23 12:01 G166_M5_featureCounts.out.summary
-rw-r--r--   1 kkarri waxmanlab 882M Apr 23 11:37 G166_M5_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 1.6G Apr 23 11:54 G166_M5_sorted.bam
-rw-r--r--   1 kkarri waxmanlab 254M Apr 23 11:37 ncRNA_exon_for_counting.gtf
==========================================================
Finished on : Tue Apr 23 12:01:44 EDT 2019
0 hours, 25 minutes and 3 seconds elapsed.
==========================================================