-----------------------
Start of variable list:
-----------------------
Sample_ID:
G166_M7
Dataset_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV
Sample_Labels_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/mouse_gtf_files/final-RNAseq-Pipeline/GTF_Files
STRANDEDNESS_featureCount:
2
FEATURE_ID:
gene_id
SCRIPT_DIR:
/net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Tue Apr 23 11:50:44 EDT 2019
Running on node : scc-kb8
Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G166_PCA_ENV/Scripts/08b_Extract_Counts_featureCounts
Current job ID : 5542352
Current job name : Step_08b_G166_M7
Task index number : undefined
Parameter for multiple cores : 16
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/5542352.1.linga

Loading required modules...


List files in scratch directory:

total 1.3G
drwx------   2 kkarri waxmanlab 4.0K Apr 23 11:51 .
drwxrwxrwt. 10 root   root      112K Apr 23 11:50 ..
-rw-r--r--   1 kkarri waxmanlab    0 Apr 23 11:51 G166_M7_featureCounts.out
-rw-r--r--   1 kkarri waxmanlab 1.3G Apr 23 11:51 G166_M7_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab  72M Apr 23 11:51 RefSeq_GeneBody.gtf

Starting to run my commands


Starting featureCounts

GTF file name: RefSeq_GeneBody
Rscript Parse_GTF.R RefSeq_GeneBody.gtf GeneSym_List.txt
[1] "Print arguments:"
[1] "-----------------"
[1] "GTF_File:"
[1] "RefSeq_GeneBody.gtf"
[1] "Subset_List:"
[1] "GeneSym_List.txt"
[1] "-----------------"
[1] "Loading RefSeq_GeneBody.gtf"
[1] "Loading GeneSym_List.txt"
[1] "Number of gene symbols in common:"
[1] "1713"
[1] "Percentage overlap:"
[1] "98%"
[1] "How many GeneSym in the GTF list and not in Subset list:"
[1] "(Expect high number)"
[1] 22484
[1] "How many GeneSym in the Subset list and not in GTF list:"
[1] "(Due to updated RefSeq gene annotations)"
[1] 27
[1] "Check out GeneSym_assign_all_features.gtf!"
[1] "GeneSym_assign_only1_feature.gtf!"
Running assign_all_features counting
featureCounts -O -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_all_features.gtf -o G166_M7_assign_all_features.out G166_M7'_sorted'.bam
Running assign_only1_feature counting
featureCounts -p -T 16 -s 2 -g gene_id -t exon -a GeneSym_assign_only1_feature.gtf -o G166_M7_assign_only1_feature.out G166_M7'_sorted'.bam
Processing G166_M7_assign_all_features.out
Done processing file.
Processing G166_M7_assign_only1_feature.out
Done processing file.

Ending featureCounts


List files in scratch

total 4.0G
drwx------  2 kkarri waxmanlab  20K Apr 23 12:35 .
drwxrwxrwt. 8 root   root      112K Apr 23 12:31 ..
-rw-r--r--  1 kkarri waxmanlab  17K Apr 23 12:35 G166_M7_assign_all_features.out
-rw-r--r--  1 kkarri waxmanlab  291 Apr 23 12:25 G166_M7_assign_all_features.out.summary
-rw-r--r--  1 kkarri waxmanlab 227K Apr 23 12:35 G166_M7_assign_only1_feature.out
-rw-r--r--  1 kkarri waxmanlab  298 Apr 23 12:35 G166_M7_assign_only1_feature.out.summary
-rw-r--r--  1 kkarri waxmanlab 244K Apr 23 12:35 G166_M7_featureCounts.out
-rw-r--r--  1 kkarri waxmanlab  654 Apr 23 12:35 G166_M7_featureCounts.out.summary
-rw-r--r--  1 kkarri waxmanlab 1.3G Apr 23 11:51 G166_M7_primary_unique.bam
-rw-r--r--  1 kkarri waxmanlab 2.6G Apr 23 12:15 G166_M7_sorted.bam
-rw-r--r--  1 kkarri waxmanlab  14K Apr 23 12:15 GeneSym_List.txt
-rw-r--r--  1 kkarri waxmanlab 1.3M Apr 23 12:15 GeneSym_assign_all_features.gtf
-rw-r--r--  1 kkarri waxmanlab  67M Apr 23 12:16 GeneSym_assign_only1_feature.gtf
-rwxr-xr-x  1 kkarri waxmanlab 6.8K Apr 23 12:15 Parse_GTF.R
-rw-r--r--  1 kkarri waxmanlab  72M Apr 23 11:51 RefSeq_GeneBody.gtf
==========================================================
Finished on : Tue Apr 23 12:35:01 EDT 2019
0 hours, 44 minutes and 17 seconds elapsed.
==========================================================