[bam_sort_core] merging from 8 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P G166_M8_sorted.bam || || || || Output file : G166_M8_assign_all_features.out || || Annotations : GeneSym_assign_all_features.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_all_features.gtf ... || || Features : 5739 || || Meta-features : 1725 || || Chromosomes/contigs : 25 || || || || Process BAM file G166_M8_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || A00296:19:H75HLDSXX:4:1101:1018:6605 89 chr17 39980205 50 || || 50M * 0 0 N # NH:i:1 || || A00296:19:H75HLDSXX:4:1101:1018:7294 89 chr11 104772184 50 || || 50M * 0 0 N # NH:i:1 || || 5640985 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 11541498 || || Successfully assigned fragments : 14162 (0.1%) || || Running time : 3.39 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================// ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P G166_M8_sorted.bam || || || || Output file : G166_M8_assign_only1_feature.out || || Annotations : GeneSym_assign_only1_feature.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file GeneSym_assign_only1_feature.gtf ... || || Features : 206368 || || Meta-features : 22472 || || Chromosomes/contigs : 32 || || || || Process BAM file G166_M8_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || A00296:19:H75HLDSXX:4:1101:1561:34538 163 chr13 115614785 5 || || 0 50M = 115614933 198 N # NH:i:1 || || A00296:19:H75HLDSXX:4:1101:1570:8187 83 chr17 39982123 50 || || 50M = 39982012 -161 N # NH:i:1 || || 5640985 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 11541498 || || Successfully assigned fragments : 9555581 (82.8%) || || Running time : 3.29 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//