[bam_sort_core] merging from 14 files... ========== _____ _ _ ____ _____ ______ _____ ===== / ____| | | | _ \| __ \| ____| /\ | __ \ ===== | (___ | | | | |_) | |__) | |__ / \ | | | | ==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | ==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | ========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ v1.4.6-p5 //========================== featureCounts setting ===========================\\ || || || Input files : 1 BAM file || || P G166_M8_sorted.bam || || || || Output file : G166_M8_featureCounts.out || || Annotations : ncRNA_exon_for_counting.gtf (GTF) || || || || Threads : 16 || || Level : meta-feature level || || Paired-end : yes || || Strand specific : inversed || || Multimapping reads : not counted || || Multi-overlapping reads : not counted || || Read orientations : fr || || || || Chimeric reads : counted || || Both ends mapped : not required || || || \\===================== http://subread.sourceforge.net/ ======================// //================================= Running ==================================\\ || || || Load annotation file ncRNA_exon_for_counting.gtf ... || || Features : 2365829 || || Meta-features : 37907 || || Chromosomes/contigs : 32 || || || || Process BAM file G166_M8_sorted.bam... || || Paired-end reads are included. || || Assign fragments (read pairs) to features... || || Found reads that are not properly paired. || || (missing mate or the mate is not the next read) || || Below are the two reads that are not properly paired: || || A00296:19:H75HLDSXX:4:1101:1018:6605 89 chr17 39980205 50 || || 50M * 0 0 N # NH:i:1 || || A00296:19:H75HLDSXX:4:1101:1018:7294 89 chr11 104772184 50 || || 50M * 0 0 N # NH:i:1 || || 8178348 reads have missing mates. || || Input was converted to a format accepted by featureCounts. || || Total fragments : 20796103 || || Successfully assigned fragments : 11771035 (56.6%) || || Running time : 5.65 minutes || || || || Read assignment finished. || || || \\===================== http://subread.sourceforge.net/ ======================//