----------------------- Need shift 5 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- SCRIPT_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170/Scripts/09b_DiffExp_3_featureCounts Dataset_DIR: /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170 Dataset_Label: G170 GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files ANNOTATION_FILE: RefSeq_GeneBody.gtf CONDITION_1_NAME: F_Placebo_TD227 CONDITION_2_NAME: F_CPA_7cycle_TD227 Lengths_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files/lengths GENE_LENGTHS_FILE: Exon_Regions_Lengths.txt COUNT_DIR: RefSeq_Exon_GTF OUTPUT_PREFIX: DiffExp_v2_GeneBody DiffExp_Index: DiffExp_3a COL_SUFFIX: GeneBody COUNT_PROGRAM: featureCounts ----------------------- End of variable list ----------------------- ========================================================== Starting on : Mon Sep 16 10:09:15 EDT 2019 Running on node : scc-kb1 Current directory : /net/waxman-server/mnt/data/waxmanlabvm_home/kkarri/G170/Scripts/09b_DiffExp_3_featureCounts Current job ID : 9373312 Current job name : Step_09b_DiffExp_3a Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/9373312.1.linga Loading required modules... ------------------------------------------ Sample_DIR: G170_M7 Sample_ID: G170_M7 Description: F_Placebo1_TD227 M_Num: M7 Copy Condition_1 sample count files to Condition_1 folder calculate mapped reads: G170_M7 Sample_DIR: G170_M8 Sample_ID: G170_M8 Description: F_Placebo2_TD227 M_Num: M8 Copy Condition_1 sample count files to Condition_1 folder calculate mapped reads: G170_M8 Sample_DIR: G170_M9 Sample_ID: G170_M9 Description: F_Placebo3_TD227 M_Num: M9 Copy Condition_1 sample count files to Condition_1 folder calculate mapped reads: G170_M9 M_Num_Cond1_List: M7M8M9 ------------------------------------------ ------------------------------------------ Sample_DIR: G170_M16 Sample_ID: G170_M16 Description: F_CPA_7cycle10_TD227 M_Num: M16 Copy Condition_2 sample count files to Condition_2 folder calculate mapped reads: G170_M16 Sample_DIR: G170_M17 Sample_ID: G170_M17 Description: F_CPA_7cycle11_TD227 M_Num: M17 Copy Condition_2 sample count files to Condition_2 folder calculate mapped reads: G170_M17 M_Num_Cond2_List: M16M17 ------------------------------------------ ========================================================== Number of replicates in each condition: NUM_REP_CONDITION1: 3 NUM_REP_CONDITION1: 3 ========================================================== Renaming input count files Counting program: Not HTSeq No lines removed. Counting program: Not HTSeq No lines removed. Counting program: Not HTSeq No lines removed. Counting program: Not HTSeq No lines removed. Counting program: Not HTSeq No lines removed. number of mapped reads 60501929 42192838 36624937 57487914 56185788 ========================================================== List files in Input -rw-r--r-- 1 kkarri waxmanlab 291522 Sep 16 10:09 /scratch/9373312.1.linga/Input/Exon_Regions_Lengths.txt -rw-r--r-- 1 kkarri waxmanlab 257784 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2270.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2270_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 255349 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2271.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2271_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 254109 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2270.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2270_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 257155 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2271.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2271_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 256961 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2272.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2272_num_mapped_reads.txt /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD227: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Sep 16 10:09 . drwxr-xr-x 4 kkarri waxmanlab 4096 Sep 16 10:09 .. /scratch/9373312.1.linga/Input/F_Placebo_TD227: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Sep 16 10:09 . drwxr-xr-x 4 kkarri waxmanlab 4096 Sep 16 10:09 .. ========================================================== Starting to run my commands Printing Rscript command: Rscript differentialAnalysis.R F_Placebo_TD227 F_CPA_7cycle_TD227 3 2 RefSeq_GeneBody.gtf /scratch/9373312.1.linga/Input DiffExp_v2_GeneBody Exon_Regions_Lengths.txt [1] "Arguments for differentialAnalysisDESeq.R:" [1] "F_Placebo_TD227" [1] "F_CPA_7cycle_TD227" [1] 3 [1] 2 [1] "RefSeq_GeneBody.gtf" [1] "/scratch/9373312.1.linga/Input" [1] "DiffExp_v2_GeneBody" [1] "Exon_Regions_Lengths.txt" [1] "sum1:214412.983148717" "sum1:218948.443771197" "sum1:210400.320518929" [4] "sum1:214587.249146281" [1] "sum2:248115.595127921" "sum2:270101.980452701" "sum2:259108.787790311" load GTF file ... parse attributes ... [1] "output file is in: /scratch/9373312.1.linga/Input/DiffExp_v2_GeneBody_F_Placebo_TD227_F_CPA_7cycle_TD227.txt" ========================================================== Create SEGEX formatted file: Printing Rscript command: Rscript formatForSegex_ver3.R DiffExp_v2_GeneBody_F_Placebo_TD227_F_CPA_7cycle_TD227.txt F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload 1 GeneBody Printing Rscript command: Rscript formatForSegex_ver4.R DiffExp_v2_GeneBody_F_Placebo_TD227_F_CPA_7cycle_TD227.txt F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload 1 GeneBody ========================================================== Comparison_Info: F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9 #---------------------------------------------------------------------------------- Running Diff_Genes.R #---------------------------------------------------------------------------------- Printing Rscript command: Rscript Diff_Genes.R F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_DESeq.txt 2 0.05 DESeq GeneBody'_'F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9 [1] "Print arguments:" [1] "-----------------" [1] "Differential expression_Output:" [1] "F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_DESeq.txt" [1] "fold_change_cutoff:" [1] "2" [1] "padj_cutoff:" [1] "0.05" [1] "postfix:" [1] "DESeq" [1] "count_method:" [1] "GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9" [1] "-----------------" [1] "Differential Gene Counts" [1] "Number of significant differential genes with positive fold change (Up Genes):" [1] 703 7 [1] "Number of significant differential genes with negative fold change (Down Genes):" [1] 546 7 [1] "Check out Up_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt!" [1] "Check out Down_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt!" Rscript Diff_Genes.R F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_EdgeR.txt 2 0.05 EdgeR GeneBody'_'F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9 [1] "Print arguments:" [1] "-----------------" [1] "Differential expression_Output:" [1] "F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_EdgeR.txt" [1] "fold_change_cutoff:" [1] "2" [1] "padj_cutoff:" [1] "0.05" [1] "postfix:" [1] "EdgeR" [1] "count_method:" [1] "GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9" [1] "-----------------" [1] "Differential Gene Counts" [1] "Number of significant differential genes with positive fold change (Up Genes):" [1] 805 7 [1] "Number of significant differential genes with negative fold change (Down Genes):" [1] 464 7 [1] "Check out Up_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt!" [1] "Check out Down_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt!" #---------------------------------------------------------------------------------- Running Venn_Diff_Genes.R #---------------------------------------------------------------------------------- Printing Rscript command: #---------------------------------------------------------------------------------- Rscript Venn_Diff_Genes.R Down_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt Down_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt GeneBody_Counting DiffExp_3a [1] "Print arguments:" [1] "-----------------" [1] "File1:" [1] "Down_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt" [1] "File2:" [1] "Down_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt" [1] "Subtitle:" [1] "GeneBody_Counting" [1] "DiffExp_Index:" [1] "DiffExp_3a" [1] "-----------------" [1] "Down.DESeq.GeneBody.HTSeq" [1] "Down.EdgeR.GeneBody.HTSeq" null device 1 [1] "Removing VennDiagram*.log files" [1] "Check out Venn diagram and Count.Table!" #---------------------------------------------------------------------------------- Rscript Venn_Diff_Genes.R Up_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt Up_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt GeneBody_Counting DiffExp_3a [1] "Print arguments:" [1] "-----------------" [1] "File1:" [1] "Up_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt" [1] "File2:" [1] "Up_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt" [1] "Subtitle:" [1] "GeneBody_Counting" [1] "DiffExp_Index:" [1] "DiffExp_3a" [1] "-----------------" [1] "Up.DESeq.GeneBody.HTSeq" [1] "Up.EdgeR.GeneBody.HTSeq" null device 1 [1] "Removing VennDiagram*.log files" [1] "Check out Venn diagram and Count.Table!" #---------------------------------------------------------------------------------- #---------------------------------------------------------------------------------- Merging Count.Table(s) into one text file #---------------------------------------------------------------------------------- Comparison_Info: F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9 #---------------------------------------------------------------------------------- ========================================================== Renaming the Differential_Expression_File ========================================================== List files in Input -rw-r--r-- 1 kkarri waxmanlab 297 Sep 16 10:10 /scratch/9373312.1.linga/Input/DiffExp_3a_Venn_Tables_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt -rw-r--r-- 1 kkarri waxmanlab 11064392 Sep 16 10:10 /scratch/9373312.1.linga/Input/DiffExp_v2_GeneBody_F_Placebo_TD227_F_CPA_7cycle_TD227.txt -rwxr-xr-x 1 kkarri waxmanlab 7394 Sep 16 10:09 /scratch/9373312.1.linga/Input/Diff_Genes.R -rw-r--r-- 1 kkarri waxmanlab 124957 Sep 16 10:10 /scratch/9373312.1.linga/Input/Down_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt -rw-r--r-- 1 kkarri waxmanlab 68649 Sep 16 10:10 /scratch/9373312.1.linga/Input/Down_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt -rw-r--r-- 1 kkarri waxmanlab 291522 Sep 16 10:09 /scratch/9373312.1.linga/Input/Exon_Regions_Lengths.txt -rw-r--r-- 1 kkarri waxmanlab 257784 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2270.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2270_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 255349 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2271.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD2271_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 6201500 Sep 16 10:10 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_DESeq.txt -rw-r--r-- 1 kkarri waxmanlab 5209423 Sep 16 10:10 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_EdgeR.txt -rw-r--r-- 1 kkarri waxmanlab 6201498 Sep 16 10:10 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_TPM_DESeq.txt -rw-r--r-- 1 kkarri waxmanlab 5209421 Sep 16 10:10 /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_TPM_EdgeR.txt -rw-r--r-- 1 kkarri waxmanlab 254109 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2270.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2270_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 257155 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2271.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2271_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 256961 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2272.out -rw-r--r-- 1 kkarri waxmanlab 9 Sep 16 10:09 /scratch/9373312.1.linga/Input/F_Placebo_TD2272_num_mapped_reads.txt -rw-r--r-- 1 kkarri waxmanlab 146211 Sep 16 10:10 /scratch/9373312.1.linga/Input/Up_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt -rw-r--r-- 1 kkarri waxmanlab 150535 Sep 16 10:10 /scratch/9373312.1.linga/Input/Up_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9.txt -rwxr-xr-x 1 kkarri waxmanlab 9553 Sep 16 10:09 /scratch/9373312.1.linga/Input/Venn_Diff_Genes.R -rw-r--r-- 1 kkarri waxmanlab 42767 Sep 16 10:10 /scratch/9373312.1.linga/Input/Venn_Down.DESeq.GeneBody.HTSeq.Down.EdgeR.GeneBody.HTSeq.png -rw-r--r-- 1 kkarri waxmanlab 40982 Sep 16 10:10 /scratch/9373312.1.linga/Input/Venn_Up.DESeq.GeneBody.HTSeq.Up.EdgeR.GeneBody.HTSeq.png -rwxr-xr-x 1 kkarri waxmanlab 3925 Sep 16 10:09 /scratch/9373312.1.linga/Input/formatForSegex_ver3.R -rwxr-xr-x 1 kkarri waxmanlab 3929 Sep 16 10:09 /scratch/9373312.1.linga/Input/formatForSegex_ver4.R /scratch/9373312.1.linga/Input/F_CPA_7cycle_TD227: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Sep 16 10:09 . drwxr-xr-x 4 kkarri waxmanlab 4096 Sep 16 10:10 .. /scratch/9373312.1.linga/Input/F_Placebo_TD227: total 8 drwxr-xr-x 2 kkarri waxmanlab 4096 Sep 16 10:09 . drwxr-xr-x 4 kkarri waxmanlab 4096 Sep 16 10:10 .. ========================================================== List files in scratch total 72M drwx------ 3 kkarri waxmanlab 4.0K Sep 16 10:10 . drwxrwxrwt. 46 root root 4.0K Sep 16 10:09 .. -rw-r--r-- 1 kkarri waxmanlab 132 Sep 16 10:09 Condition_1.txt -rw-r--r-- 1 kkarri waxmanlab 111 Sep 16 10:09 Condition_2.txt drwxr-xr-x 4 kkarri waxmanlab 4.0K Sep 16 10:10 Input -rw-r--r-- 1 kkarri waxmanlab 72M Sep 16 10:09 RefSeq_GeneBody.gtf -rwxr-xr-x 1 kkarri waxmanlab 17K Sep 16 10:09 differentialAnalysis.R ========================================================== Re-naming files in OUTPUT_DIR Need to append the COUNT_PROGRAM name to all output files List files in OUTPUT_DIR total 66M drwxr-xr-x 2 kkarri waxmanlab 4.0K Sep 16 10:05 . drwxr-xr-x 6 kkarri waxmanlab 4.0K Sep 16 10:03 .. -rw-r--r-- 1 kkarri waxmanlab 297 Sep 16 10:05 DiffExp_3a_Venn_Tables_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 11M Sep 16 10:05 DiffExp_v2_GeneBody_F_Placebo_TD227_F_CPA_7cycle_TD227_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 123K Sep 16 10:05 Down_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 68K Sep 16 10:05 Down_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 6.0M Sep 16 10:05 F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_DESeq_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 5.0M Sep 16 10:05 F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_EdgeR_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 6.0M Sep 16 10:05 F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_TPM_DESeq_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 5.0M Sep 16 10:05 F_CPA_7cycle_TD227_G170_M16M17_vs_F_Placebo_TD227_G170_M7M8M9_DiffExp_v2_GeneBody_forSEGEXUpload_TPM_EdgeR_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 143K Sep 16 10:05 Up_Genes_DESeq_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 148K Sep 16 10:05 Up_Genes_EdgeR_GeneBody_F_CPA_7cycle_TD227_G170_M16M17_F_Placebo_TD227_G170_M7M8M9_featureCounts.txt -rw-r--r-- 1 kkarri waxmanlab 42K Sep 16 10:05 Venn_Down.DESeq.GeneBody.HTSeq.Down.EdgeR.GeneBody.HTSeq_featureCounts.png -rw-r--r-- 1 kkarri waxmanlab 41K Sep 16 10:05 Venn_Up.DESeq.GeneBody.HTSeq.Up.EdgeR.GeneBody.HTSeq_featureCounts.png ========================================================== ========================================================== Finished on : Mon Sep 16 10:10:41 EDT 2019 1 minutes and 26 seconds elapsed. ==========================================================