----------------------- Need shift 1 command (have more than 9 arguments): ----------------------- ----------------------- Start of variable list: ----------------------- Sample_ID: G167_M2 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples/Scripts/00_Setup_Pipeline GTF_Files_DIR: /unprotected/projects/waxmanlab/routines/GTF_Files MODE: intersection-nonempty STRANDEDNESS_HTSeq: reverse FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples/Scripts/08a_Extract_Counts_HTSeq ANNOTATION_FILE: genes.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Wed Jul 18 13:42:51 EDT 2018 Running on node : scc-tl1 Current directory : /restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples/Scripts/08a_Extract_Counts_HTSeq Current job ID : 6966598 Current job name : Step_08a_G167_M2 Task index number : undefined Parameter for multiple cores : 1 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/6966598.1.linga Loading required modules... List files in scratch directory: total 987M drwx------ 2 kkarri waxmanlab 4.0K Jul 18 13:42 . drwxrwxrwt. 28 root root 20K Jul 18 13:42 .. -rw-r--r-- 1 kkarri waxmanlab 0 Jul 18 13:42 G167_M2_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 907M Jul 18 13:42 G167_M2_primary_unique.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Jul 18 13:42 genes.gtf Starting to run my commands Starting samtools and htseq-count samtools view G167_M2'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf > G167_M2_HTSeq.out Ending samtools and htseq-count List files in scratch total 2.7G drwx------ 2 kkarri waxmanlab 4.0K Jul 18 13:52 . drwxrwxrwt. 33 root root 20K Jul 18 13:51 .. -rw-r--r-- 1 kkarri waxmanlab 0 Jul 18 13:52 G167_M2_HTSeq.out -rw-r--r-- 1 kkarri waxmanlab 907M Jul 18 13:42 G167_M2_primary_unique.bam -rw-r--r-- 1 kkarri waxmanlab 1.8G Jul 18 13:52 G167_M2_sorted.bam -rwxr-xr-x 1 kkarri waxmanlab 81M Jul 18 13:42 genes.gtf ========================================================== Finished on : Wed Jul 18 13:52:37 EDT 2018 0 hours, 9 minutes and 46 seconds elapsed. ==========================================================