-----------------------
Need shift 1 command (have more than 9 arguments):
-----------------------
-----------------------
Start of variable list:
-----------------------
Sample_ID:
G167_M6
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/unprotected/projects/waxmanlab/routines/GTF_Files
MODE:
intersection-nonempty
STRANDEDNESS_HTSeq:
reverse
FEATURE_ID:
gene_id
SCRIPT_DIR:
/restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples/Scripts/08a_Extract_Counts_HTSeq
ANNOTATION_FILE:
RefSeq_GeneBody.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Wed Jul 18 13:43:14 EDT 2018
Running on node : scc-tk4
Current directory : /restricted/projectnb/waxmanlab/kkarri/Chad/G167/G167_Samples/Scripts/08a_Extract_Counts_HTSeq
Current job ID : 6966613
Current job name : Step_08a_G167_M6
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/6966613.1.linga

Loading required modules...


List files in scratch directory:

total 727M
drwx------   2 kkarri waxmanlab 4.0K Jul 18 13:43 .
drwxrwxrwt. 40 root   root       36K Jul 18 13:43 ..
-rw-r--r--   1 kkarri waxmanlab    0 Jul 18 13:43 G167_M6_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 656M Jul 18 13:43 G167_M6_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab  72M Jul 18 13:43 RefSeq_GeneBody.gtf

Starting to run my commands


Starting samtools and htseq-count

samtools view G167_M6'_sorted'.bam | htseq-count --stranded=reverse --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - RefSeq_GeneBody.gtf  > G167_M6_HTSeq.out

Ending samtools and htseq-count


List files in scratch

total 2.1G
drwx------   2 kkarri waxmanlab 4.0K Jul 18 13:50 .
drwxrwxrwt. 40 root   root       36K Jul 18 13:44 ..
-rw-r--r--   1 kkarri waxmanlab    0 Jul 18 13:50 G167_M6_HTSeq.out
-rw-r--r--   1 kkarri waxmanlab 656M Jul 18 13:43 G167_M6_primary_unique.bam
-rw-r--r--   1 kkarri waxmanlab 1.4G Jul 18 13:50 G167_M6_sorted.bam
-rw-r--r--   1 kkarri waxmanlab  72M Jul 18 13:43 RefSeq_GeneBody.gtf
==========================================================
Finished on : Wed Jul 18 13:50:39 EDT 2018
0 hours, 7 minutes and 25 seconds elapsed.
==========================================================