-----------------------
Need shift 1 command (have more than 9 arguments):
-----------------------
-----------------------
Start of variable list:
-----------------------
Sample_ID:
IS_M2
Dataset_DIR:
/restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline
Sample_Labels_DIR:
/restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/00_Setup_Pipeline
GTF_Files_DIR:
/restricted/projectnb/waxmanlab/kkarri/GTF_Files1
MODE:
intersection-nonempty
STRANDEDNESS_HTSeq:
no
FEATURE_ID:
gene_id
SCRIPT_DIR:
/restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/08a_Extract_Counts_HTSeq
ANNOTATION_FILE:
genes.gtf
FEATURE_TYPE:
exon
-----------------------
End of variable list
-----------------------
==========================================================
Starting on : Sat Jun 17 05:06:48 EDT 2017
Running on node : scc-tk2
Current directory : /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/08a_Extract_Counts_HTSeq
Current job ID : 9394989
Current job name : Step_08a_IS_M2
Task index number : undefined
Parameter for multiple cores : 1
==========================================================

Change dir to scratch directory


Print scratch directory location:

/scratch/9394989.1.linga

Loading required modules...


List files in scratch directory:

total 2.1G
drwxr-xr-x  2 kkarri montilab-p 4.0K Jun 17 05:07 .
drwxrwxrwt. 9 root   root        36K Jun 17 05:06 ..
-rw-r--r--  1 kkarri montilab-p    0 Jun 17 05:07 IS_M2_HTSeq.out
-rw-r--r--  1 kkarri montilab-p 2.0G Jun 17 05:07 IS_M2_primary_unique.bam
-rwxr-xr-x  1 kkarri montilab-p  52M Jun 17 05:07 genes.gtf

Starting to run my commands


Starting samtools and htseq-count

samtools view IS_M2'_sorted'.bam | htseq-count --stranded=no --type=exon --idattr=gene_id --order=name --mode=intersection-nonempty - genes.gtf  > IS_M2_HTSeq.out

Ending samtools and htseq-count


List files in scratch

total 5.0G
drwxr-xr-x  2 kkarri montilab-p 4.0K Jun 17 05:19 .
drwxrwxrwt. 8 root   root        36K Jun 17 05:37 ..
-rw-r--r--  1 kkarri montilab-p 165K Jun 17 05:40 IS_M2_HTSeq.out
-rw-r--r--  1 kkarri montilab-p 2.0G Jun 17 05:07 IS_M2_primary_unique.bam
-rw-r--r--  1 kkarri montilab-p 2.9G Jun 17 05:19 IS_M2_sorted.bam
-rwxr-xr-x  1 kkarri montilab-p  52M Jun 17 05:07 genes.gtf
==========================================================
Finished on : Sat Jun 17 05:40:24 EDT 2017
0 hours, 33 minutes and 36 seconds elapsed.
==========================================================