----------------------- Start of variable list: ----------------------- Sample_ID: IS_M1 Dataset_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline Sample_Labels_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/00_Setup_Pipeline GTF_Files_DIR: /restricted/projectnb/waxmanlab/kkarri/GTF_Files1 STRANDEDNESS_featureCount: 0 FEATURE_ID: gene_id SCRIPT_DIR: /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/08b_Extract_Counts_featureCounts ANNOTATION_FILE: RefSeq_GeneBody.gtf FEATURE_TYPE: exon ----------------------- End of variable list ----------------------- ========================================================== Starting on : Sat Jun 17 11:21:25 EDT 2017 Running on node : scc-pf5 Current directory : /restricted/projectnb/waxmanlab/kkarri/samples/fastqfiles/Water_Saline/Scripts/08b_Extract_Counts_featureCounts Current job ID : 9395303 Current job name : Step_08b_IS_M1 Task index number : undefined Parameter for multiple cores : 16 ========================================================== Change dir to scratch directory Print scratch directory location: /scratch/9395303.1.p8 Loading required modules... List files in scratch directory: total 1.7G drwxr-xr-x 2 kkarri montilab-p 4.0K Jun 17 11:21 . drwxrwxrwt. 24 root root 4.0K Jun 17 11:21 .. -rw-r--r-- 1 kkarri montilab-p 0 Jun 17 11:21 IS_M1_featureCounts.out -rw-r--r-- 1 kkarri montilab-p 1.6G Jun 17 11:21 IS_M1_primary_unique.bam -rwx------ 1 kkarri montilab-p 41M Jun 17 11:21 RefSeq_GeneBody.gtf Starting to run my commands Starting featureCounts GTF file name: RefSeq_GeneBody Rscript Parse_GTF.R RefSeq_GeneBody.gtf GeneSym_List.txt [1] "Print arguments:" [1] "-----------------" [1] "GTF_File:" [1] "RefSeq_GeneBody.gtf" [1] "Subset_List:" [1] "GeneSym_List.txt" [1] "-----------------" [1] "Loading RefSeq_GeneBody.gtf" [1] "Loading GeneSym_List.txt" Running assign_all_features counting featureCounts -O -p -T 16 -s 0 -g gene_id -t exon -a GeneSym_assign_all_features.gtf -o IS_M1_assign_all_features.out IS_M1'_sorted'.bam Running assign_only1_feature counting featureCounts -p -T 16 -s 0 -g gene_id -t exon -a GeneSym_assign_only1_feature.gtf -o IS_M1_assign_only1_feature.out IS_M1'_sorted'.bam Processing *_assign_*.out Done processing file. Ending featureCounts List files in scratch total 3.9G -rw-r--r-- 1 kkarri montilab-p 0 Jun 17 11:30 *_assign_*.out drwxr-xr-x 2 kkarri montilab-p 4.0K Jun 17 11:30 . drwxrwxrwt. 24 root root 4.0K Jun 17 11:21 .. -rw-r--r-- 1 kkarri montilab-p 0 Jun 17 11:30 IS_M1_featureCounts.out -rw-r--r-- 1 kkarri montilab-p 61 Jun 17 11:30 IS_M1_featureCounts.out.summary -rw-r--r-- 1 kkarri montilab-p 1.6G Jun 17 11:21 IS_M1_primary_unique.bam -rw-r--r-- 1 kkarri montilab-p 2.3G Jun 17 11:30 IS_M1_sorted.bam -rwxr-xr-x 1 kkarri montilab-p 6.8K Jun 17 11:30 Parse_GTF.R -rwx------ 1 kkarri montilab-p 41M Jun 17 11:21 RefSeq_GeneBody.gtf ========================================================== Finished on : Sat Jun 17 11:30:50 EDT 2017 0 hours, 9 minutes and 25 seconds elapsed. ==========================================================